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Literature summary for 3.1.21.3 extracted from

  • Carpenter, M.A.; Bhagwat, A.S.
    DNA base flipping by both members of the PspGI restriction-modification system (2008), Nucleic Acids Res., 36, 5417-5425.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
wild-type R.PspGI and its mutant expressed as pET21a-PspGI-WT and pET21a-PspGI-D138A in Escherichia coli strain ER2744 Pyrococcus sp.

Protein Variants

Protein Variants Comment Organism
D138A catalytically inactive Pyrococcus sp.

Organism

Organism UniProt Comment Textmining
Pyrococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type R.PspGI and its mutant purified by chromatography, to homogeneity Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the PspGI restriction-modification system recognizes the sequence CCWGG. R.PspGI cuts DNA before the first C in the cognate sequence and M.PspGI methylates N4 of one of the cytosines in the sequence. R.PspGI flips both bases in the central base pair out of the duplex Pyrococcus sp. ?
-
?

Synonyms

Synonyms Comment Organism
PspGI endonuclease
-
Pyrococcus sp.
PspGI restriction-modification system
-
Pyrococcus sp.
R.PspGI
-
Pyrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
optimal temperature for DNA cleavage. Optimal flipping occurs at temperatures substantially below the growth temperature of the source organism for PspGI and for the catalytic activity of endonuclease Pyrococcus sp.