Application | Comment | Organism |
---|---|---|
medicine | identification of a putative restriction-modification system EcoA0ORF42P in the commensal Escherichia coli strain A0 34/86 (O83: K24: H31), which is efficiently used at Czech paediatric clinics for prophylaxis and treatment of nosocomial infections and diarrhoea of preterm and newborn infants. This type I R-M system is a member of the type IB family, but is not an isoschizomer of either any prototype of the type IB members or any sequenced putative IB R-M systems. It is designated as EcoAO83I, the DNA recognition sequence of the EcoAO83I is GGA(8N)ATGC. Combination of the classical biochemical and bacterial genetics approaches with comparative genomics may contribute effectively to further classification of many other putative type-I enzymes, especially in clinical samples | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
transforming the Escherichia coli BL21(DE3) strains with a BAC C4/1 carrying the hsdR, hsdM and hsdS genes of EcoAO83I and with plasmids carrying the hsdS and hsdM genes of EcoAI | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | Q07736 | - |
- |
Escherichia coli | Q47281 | - |
- |
Escherichia coli A0 34/86 (O83:K24:H31) | - |
- |
- |
Escherichia coli A0 34/86 (O83:K24:H31) | Q07736 | - |
- |
Escherichia coli A0 34/86 (O83:K24:H31) | Q47281 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
duplex DNA + ATP | - |
Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? | |
duplex DNA + ATP | - |
Escherichia coli A0 34/86 (O83:K24:H31) | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EcoA0ORF42P | - |
Escherichia coli |
EcoAI | - |
Escherichia coli |
EcoAO83I | - |
Escherichia coli |
EcoEI | - |
Escherichia coli |
type I R-M system | - |
Escherichia coli |
type I restriction-modification system | - |
Escherichia coli |
type IB restriction enzyme | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |