Literature summary for 3.1.21.3 extracted from

Lee, H.J.; Nishi, K.; Song, J.M.; Kim, J.S.
Expression, crystallization and preliminary X-ray diffraction analysis of a modification subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016 (2009), Acta Crystallogr. Sect. F, 65, 1271-1273.

Search for more literature for 3.1.21.3

Cloned(Commentary)

Cloned (Comment)	Organism
putative HsdM subunit amplified, cloned into pProExHTc, which expresses 25 extra amino acids containing six consecutive His residues at the N-terminus. The expression construct transformed into Escherichia coli B834 (DE3)	Vibrio vulnificus YJ016

Crystallization (Commentary)

Crystallization (Comment)	Organism
HsdM subunit, to 1.86 A resolution, by hanging-drop and sitting-drop vapour-diffusion methods. Crystal belongs to the tetragonal space group P41212 or P43212, with unit-cell parameters a = b = 78.9, c = 165.8 A. With one molecule in the asymmetric unit, the crystal volume per unit protein weight is 2.12 A3/Da, with a solvent content of 42%	Vibrio vulnificus YJ016

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	x * 60000, HsdM subunit, crystallography	Vibrio vulnificus YJ016

Organism

Organism	UniProt	Comment	Textmining
Vibrio vulnificus YJ016	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HsdM subunit, on anion-exchange column, more than 95% pure	Vibrio vulnificus YJ016

Subunits

Subunits	Comment	Organism
?	x * 60000, HsdM subunit, crystallography	Vibrio vulnificus YJ016

Synonyms

Synonyms	Comment	Organism
type I restriction enzyme	-	Vibrio vulnificus YJ016

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Release 2023.1 (January 2023)