Literature summary for 3.1.21.3 extracted from

Grinkevich, P.; Iermak, I.; Luedtke, N.A.; Mesters, J.R.; Ettrich, R.; Ludwig, J.
pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I (2016), Acta Crystallogr. Sect. F, 72, 672-676 .

Cloned(Commentary)

Cloned (Comment)	Organism
pHluorin-assisted expression of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting-drop vapour-diffusion technique, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I. The shortest of the three encompassed HsdR residues 887-1038 and yielded crystals that belonged to the orthorhombic space group C2221, with unit-cell parameters a = 83.42, b = 176.58, c = 126.03 A, alpha = beta = gamma = 90.0 and two molecules in the asymmetric unit. X-ray diffraction data are collected to a resolution of 2.45 A	Escherichia coli

Crystallization (Comment)

Organism

sitting-drop vapour-diffusion technique, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I. The shortest of the three encompassed HsdR residues 887-1038 and yielded crystals that belonged to the orthorhombic space group C2221, with unit-cell parameters a = 83.42, b = 176.58, c = 126.03 A, alpha = beta = gamma = 90.0 and two molecules in the asymmetric unit. X-ray diffraction data are collected to a resolution of 2.45 A

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q304R3	HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I	-

Purification (Commentary)

Purification (Comment)	Organism
pHluorin-assisted purification of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I	Escherichia coli

Subunits

Subunits	Comment	Organism
More	the HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits	Escherichia coli

Synonyms

Synonyms	Comment	Organism
type I restriction-modification system EcoR124I	-	Escherichia coli