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Literature summary for 3.1.26.3 extracted from

  • Gross, G.; Dunn, J.J.
    Structure of secondary cleavage sites of E. coli RNAaseIII in A3t RNA from bacteriophage T7 (1987), Nucleic Acids Res., 15, 431-442.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Inhibitors

Inhibitors Comment Organism Structure
NaCl 0.15-0.3 M cleaves at primary cleavage sites, inhibition at higher concentrations, at 5 mM additional secondary cleavage sites Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ different cleavage pattern depending on the cation used Escherichia coli
Mn2+ different cleavage pattern depending on the cation used Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Escherichia coli cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA 5'-phosphooligonucleotides
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O
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 Escherichia coli 5'-phosphooligonucleotides 15 bases in average ?
double-stranded RNA + H2O cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA Escherichia coli 5'-phosphooligonucleotides
-
 ?
tRNA + H2O five additional secondary cleavage sites in RNA A3t from bacteriophage T7 Escherichia coli 5'-phosphooligonucleotides
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 ?