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Literature summary for 3.1.26.3 extracted from
- The N-terminal domain that distinguishes yeast from bacterial RNase III contains a dimerization signal required for efficient double-stranded RNA cleavage (2000), Mol. Cell. Biol., 20, 1104-1115.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of N-terminal and dsRNA binding domain in a two-hybrid system, expression of His-tagged wild-type enzyme and deletion mutants in Escherichia coli BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of several deletion mutants, lacking parts or total of the C-terminus or N-terminus, deletion of the N-terminal domain leads to slight accumulation of unprocessed 25S pre-rRNA in vivo and reduced enzyme activity in vitro | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 25S pre-rRNA + H2O | Saccharomyces cerevisiae | processing | 25S rRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 25S pre-rRNA + H2O | - |
Saccharomyces cerevisiae | 25S rRNA | - |
? | |
| 25S pre-rRNA + H2O | processing | Saccharomyces cerevisiae | 25S rRNA | - |
? | |
| dsRNA + H2O | specific for double-stranded RNA, a dimerization signal within the N-terminal domain is required for efficient cleavage | Saccharomyces cerevisiae | mature RNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme contains the conserved dsRNA binding and nuclease domains, the dsRBD self-interacts with the N-terminal domain to stabilize the enzyme homodimer | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase III | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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