Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) as C-terminally His-tagged proteins | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of deletion mutants RNT1DELTA2-329 and RNT1DELTA2-198, which are both catalytically active in vitro but do not rescue a growth defective mutant and are not able to retain activity and viability in vivo, construction of a AGNN-loop exchange mutant GNRA-loop shows reduced activity and substrate selectivity | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, amino acid sequence calculation | Saccharomyces cerevisiae |
110000 | - |
recombinant wild-type enzyme, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-rRNA + H2O | Saccharomyces cerevisiae | enzyme is required for processing | mature rRNA | - |
? | |
pre-snoRNA + H2O | Saccharomyces cerevisiae | enzyme is required for processing | mature snoRNA | - |
? | |
pre-snRNA + H2O | Saccharomyces cerevisiae | enzyme is required for processing | mature snRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli, expression of the mutant enzymes in a two-hybrid system in Saccharomyces strains | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Endonucleolytic cleavage to a 5'-phosphomonoester | RNA substrate forms a duplex structure with a terminal four-base loop with the sequence AGNN, being an important determinant of the substrate selectivity in the dsRNA binding domain dsRBD, the N-terminal region is not required for selective cleavage in vitro but required for full function and viability of yeast cells in vivo, substrate binding modeling | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-rRNA + H2O | - |
Saccharomyces cerevisiae | mature rRNA | - |
? | |
pre-rRNA + H2O | enzyme is required for processing | Saccharomyces cerevisiae | mature rRNA | - |
? | |
pre-snoRNA + H2O | - |
Saccharomyces cerevisiae | mature snoRNA | - |
? | |
pre-snoRNA + H2O | enzyme is required for processing | Saccharomyces cerevisiae | mature snoRNA | - |
? | |
pre-snRNA + H2O | - |
Saccharomyces cerevisiae | mature snRNA | - |
? | |
pre-snRNA + H2O | enzyme is required for processing | Saccharomyces cerevisiae | mature snRNA | - |
? | |
synthetic 25S rRNA 3' ETS cleavage site containing RNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, amino acid sequence calculation | Saccharomyces cerevisiae |
More | deletion mutant RNT1DELTA2-198 also behaves as a dimer | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
RNase III | - |
Saccharomyces cerevisiae |
RNT1 | - |
Saccharomyces cerevisiae |
Rnt1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |