Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.3 extracted from

  • Takeshita, D.; Zenno, S.; Lee, W.C.; Nagata, K.; Saigo, K.; Tanokura, M.
    Homodimeric structure and double-stranded RNA cleavage activity of the C-terminal RNase III domain of human dicer (2007), J. Mol. Biol., 374, 106-120.
    View publication on PubMed
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tag fused RNase IIIb-dsRBD fragment of human Dicer (residues 1649-1922) in Escherichia coli HT115 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant RNase IIIb-dsRBD fragment by sitting drop vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
D1709A strongly reduced dsRNA cleavage activity Homo sapiens
D1713A no significant effect on the cleavage activity Homo sapiens
D1713K no significant effect on the cleavage activity Homo sapiens
D1810A reduced dsRNA cleavage activity Homo sapiens
DELTA1787-1799 no significant effect on the cleavage activity Homo sapiens
E1705A reduced dsRNA cleavage activity Homo sapiens
E1813A strongly reduced dsRNA cleavage activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ two ions bound around the active site, one of which is involved in the catalytic mechanism and the second in RNA substrate binding Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Homo sapiens responsible for the production of short interfering RNAs and microRNAs that induce gene silencing known as RNA interference 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UPY3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O responsible for the production of short interfering RNAs and microRNAs that induce gene silencing known as RNA interference Homo sapiens 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O 114 bp in length Homo sapiens 5'-phosphooligonucleotides products shorter than 21 bp ?

Subunits

Subunits Comment Organism
dimer recombinant RNase IIIb-dsRBD fragment, crystal structure analysis, and gel filtration data Homo sapiens

Synonyms

Synonyms Comment Organism
Dicer Dicer contains two RNase III domains (RNase IIIa and RNase IIIb) Homo sapiens