Organism | UniProt | Comment | Textmining |
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Saccharomyces cerevisiae | Q02555 | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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additional information | design of series of bipartite substrates permitting the distinction between binding and cleavage defects. Each substrate is engineered to carry a single or multiple 2'-O-methyl or 2'-fluoro ribonucleotide substitutions to prevent the formation of hydrogen bonds with a specific nucleotide or group of nucleotides. Introduction of 2'-O-methyl ribonucleotides near the cleavage site increases the rate of catalysis, indicating that 2'-OH are not required for cleavage. Substitution of nucleotides in known Rnt1p binding site with 2'-O-methyl ribonucleotides inhibits cleavage while single 2'-fluoro ribonucleotide substitutions does not. This indicates that while no single 2'-OH is essential for Rnt1p cleavage, small changes in the substrate structure are not tolerated | Saccharomyces cerevisiae | ? | - |
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Synonyms | Comment | Organism |
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ribonuclease III | - |
Saccharomyces cerevisiae |
Rnt1p | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
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additional information | - |
additional information | Rnt1p cleavage of bipartite substrates: 0.087 1/s for EL11:TL as a substrate, 0.248 1/s for EL18-EM:TL as a substrate, 0.55 1/s for EL11-EM:TL as a substrate, 0.302 1/s for EL11-EM+1:TL as a substrate, 0.4 1/s for EL11-5'3'M:TL as a substrate, 0.55 1/s for EL11-dT:TL as a substrate, 0.028 1/s for EL11-ES:TL as a substrate, 0.042 1/s for EL11-5'stemM:TL as a substrate, 0.03 1/s for EL11-A6M:TL as a substrate. Rnt1p cleavage of 2'-fluoro-modified substrate: 0.07 1/s for EL11:TL as a substrate, 0.0283 1/s for EL11-A6F:TL as a substrate, 0.026 1/s for EL11-G7F:TL as a substrate, 0.016 1/s for EL11-C10F:TL as a substrate, 0.026 1/s for EL11-AAGU:TL as a substrate, 0.0115 1/s for EL11-AAGU-A6F:TL as a substrate, 0.013 1/s for EL11-AAGU-A7F:TL as a substrate, 0.0078 1/s for EL11-AAGU-C10F:TL as a substrate, 0.0183 1/s for EL11:TL as a substrate. DELTAN-term cleavage of 2'-fluoro-modified substrate: 0.022 1/s for EL11-A6F:TL as a substrate, 0.012 1/s for EL11-G7F:TL as a substrate, 0.012 1/s for EL11-C10F:TL as a substrate, 0.01 1/s for EL11-AAGU:TL as a substrate, 0.0085 1/s for EL11-AAGU-A6F:TL as a substrate, 0.0073 1/s for EL11-AAGU-A7F:TL as a substrate, 0.00783 1/s for EL11-AAGU-C10F:TL as a substrate | Saccharomyces cerevisiae |