Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | silencing of the drosha cofactor, pasha, leads to developmental complications and consequent lethality | Meloidogyne incognita |
Application | Comment | Organism |
---|---|---|
additional information | drosha, pasha and their ancillary factors may represent excellent targets for novel nematicides and/or in planta controls, and potentially other parasitic nematodes, through disruption of micro-RNA-directed developmental pathways | Meloidogyne incognita |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | dicer | Meloidogyne incognita | 5829 | - |
nucleus | drosha | Meloidogyne incognita | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meloidogyne incognita | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Meloidogyne incognita | - |
Synonyms | Comment | Organism |
---|---|---|
Dicer | - |
Meloidogyne incognita |
Drosha | - |
Meloidogyne incognita |
RNase III | - |
Meloidogyne incognita |
Organism | Comment | Expression |
---|---|---|
Meloidogyne incognita | Micro-drosha siRNAs-1 to -3 yield a very highly significant level of target transcript knockdown compared with the control siRNA following egg-stage soaks, with a mean 88.1%, 98.6%, and 99.89% decrease, respectively. Soaking the J2s in dicer siRNA results in a modest decrease in dicer transcript abundance | down |
Meloidogyne incognita | Micro-dicer siRNAs-1 to -3 elicit percentage transcript increases of 775, 1832 and 645, respectively | up |
General Information | Comment | Organism |
---|---|---|
malfunction | on silencing drosha, a critical effector of micro-RNA maturation, significant inhibition of normal development and hatching in short interfering (si)RNA-soaked eggs. Drosha knockdown proves embryonically lethal. Impact of silencing dicer substantial up-regulates dicer transcript abundance, which does not impact on egg differentiation or hatching rates. Soaking the J2s in dicer siRNA results in a modest decrease in dicer transcript abundance which has no observable impact on phenotype or behaviour within 48 h of initial exposure to siRNA | Meloidogyne incognita |