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Literature summary for 3.1.26.3 extracted from

  • Tang, X.; Zhang, Y.; Tucker, L.; Ramratnam, B.
    Phosphorylation of the RNase III enzyme Drosha at serine 300 or serine 302 is required for its nuclear localization (2010), Nucleic Acids Res., 38, 6610-6619.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged wild-type and mutant enzymes in nuclei of HeLa, HEK293T, and Huh-7 cells Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
S300A site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
S300A/S302A site-directed mutagenesis, the double mutation completely disrupts nuclear localization Drosophila melanogaster
S300E site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
S300E/S302D site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
S302A site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
S302D site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus phosphorylation at Ser300 or Ser302 locates Drosha to the nucleus. Nuclear localization of Drosha is critical for its functionality in miRNA processing Drosophila melanogaster 5634
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Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
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-
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Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein two phosphorylation sites at Ser300 and Ser302, which locates Drosha to the nucleus Drosophila melanogaster

Subunits

Subunits Comment Organism
More the RNA binding and enzymatic domains of Drosha are located on its C-terminus, the N-terminus harbors a nuclear localization signal Drosophila melanogaster

Synonyms

Synonyms Comment Organism
Drosha
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Drosophila melanogaster
RNase III
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Drosophila melanogaster

General Information

General Information Comment Organism
additional information the RNA binding and enzymatic domains of Drosha are located on its C-terminus, the N-terminus harbors a nuclear localization signal Drosophila melanogaster
physiological function the RNaseIII enzyme Drosha plays a pivotal role in microRNA, miRNA, biogenesis by cleaving primary miRNA transcripts to generate precursor miRNA in the nucleus. Nuclear localization of Drosha is critical for its functionality in miRNA processing Drosophila melanogaster