Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged wild-type and mutant enzymes in nuclei of HeLa, HEK293T, and Huh-7 cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
S300A | site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme | Drosophila melanogaster |
S300A/S302A | site-directed mutagenesis, the double mutation completely disrupts nuclear localization | Drosophila melanogaster |
S300E | site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme | Drosophila melanogaster |
S300E/S302D | site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme | Drosophila melanogaster |
S302A | site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme | Drosophila melanogaster |
S302D | site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | phosphorylation at Ser300 or Ser302 locates Drosha to the nucleus. Nuclear localization of Drosha is critical for its functionality in miRNA processing | Drosophila melanogaster | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | two phosphorylation sites at Ser300 and Ser302, which locates Drosha to the nucleus | Drosophila melanogaster |
Subunits | Comment | Organism |
---|---|---|
More | the RNA binding and enzymatic domains of Drosha are located on its C-terminus, the N-terminus harbors a nuclear localization signal | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
Drosha | - |
Drosophila melanogaster |
RNase III | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
additional information | the RNA binding and enzymatic domains of Drosha are located on its C-terminus, the N-terminus harbors a nuclear localization signal | Drosophila melanogaster |
physiological function | the RNaseIII enzyme Drosha plays a pivotal role in microRNA, miRNA, biogenesis by cleaving primary miRNA transcripts to generate precursor miRNA in the nucleus. Nuclear localization of Drosha is critical for its functionality in miRNA processing | Drosophila melanogaster |