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Literature summary for 3.1.26.3 extracted from

  • Lien, P.C.; Kuo, P.H.; Chen, C.J.; Chang, H.H.; Fang, S.L.; Wu, W.S.; Lai, Y.K.; Pai, T.W.; Chang, M.D.
    In silico prediction and in vitro characterization of multifunctional human RNase3 (2013), BioMed Res. Int., 2013, 170398.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli BL21(DE3) Codon Plus Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of mutant enzymes containing inserts encoding the enzyme's heparin binding region HBR1, HBR2, or HBR3 by site-directed mutagenesis, cytotoxicity of wild-type and mutant enzymes to Beas-2B cells, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme interacts with membrane lipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) Codon Plus by metal affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
BEAS-2B cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme interacts with membrane lipids Homo sapiens ?
-
 ?
additional information enzyme assay with commercial yeast tRNA Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase3
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information three putative multifunctional heparin binding regions, 34RWRCK38 (HBR1), 75RSRFR79 (HBR2), and 101RPGRR105 (HBR3), of hRNase3 are identified by silico sequence analysis and validated by in vitro activity assays, the heparin binding peptide containing HBR1 is a key element associated with heparan sulfate binding, cellular binding, and lipid binding activities. Comparisons of CHO cell binding and cytotoxicity to Beas-2B cells of wild-type and mutant enzymes, overview Homo sapiens
physiological function the human enzyme has multiple functions including ribonucleolytic, heparan sulfate binding, cellular binding, endocytic, lipid destabilization, cytotoxic, and antimicrobial activities Homo sapiens