Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 100 mM, isoenzyme I and II are activated by 50%. Isoenzyme II shows optimal activity at 50 mM, 80% inhibition at 200 mM | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | isoenzyme II, 80% inhibition at 200 mM | Rattus norvegicus | |
PCMB | in absence of 2-mercaptoethanol, isoenzyme I and III are completely inhibited by 0.2 mM PCMB, Activity of isoenzyme II is inhibited 20% | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | isoenzyme I and II both require 10-15 mM Mg2+ for maximal activity. Isoenzyme II is maximally activated by Mg2+, some activity with Mn2+ | Rattus norvegicus | |
Mn2+ | isoenzyme II is maximally active at 0.4 mM, some activity with Mg2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme I, II and III | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | poly(rAdT) | Rattus norvegicus | ribonucleotide 5'-phosphomonoester | oligonucleotides with 3'-hydroxyl and 5'-phosphate termini with the structure (pA)3-9 are formed from poly(A)*poly(dT) | ? |