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Literature summary for 3.1.26.4 extracted from

  • Frank, P.; Albert, S.; Cazenave, C.; Toulme, J.J.
    Purification and characterization of human ribonuclease HI (1994), Nucleic Acids Res., 22, 5247-5254.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Inhibitors

Inhibitors Comment Organism Structure
Co2+ in presence of Mg2+, inhibition Homo sapiens
Cu2+ in presence of Mg2+, inhibition Homo sapiens
Fe2+ in presence of Mg2+, inhibition Homo sapiens
Mn2+ in presence of Mg2+, strong inhibition Homo sapiens
NEM 2 mM, 50% inhibition Homo sapiens
Zn2+ in presence of Mg2+, inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 DNA/RNA hybrid obtained by transcription of calf thymus DNA Homo sapiens
0.0016
-
 poly(rAdT)
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ broad optimum around 20 mM Homo sapiens
Mn2+ slightly active with Mn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA/RNA hybrid + H2O
-
 Homo sapiens ?
-
 ?
poly(rAdT) + H2O
-
 Homo sapiens ?
-
 ?
RNA-DNA hybrid + H2O hybrid obtained by transcription of calf thymus DNA Homo sapiens ribonucleotide 5'-phosphomonoester oligoribonucleotides with 3'-OH and 5'-phosphate ends ?
RNA-DNA hybrid + H2O poly(rAdT) Homo sapiens ribonucleotide 5'-phosphomonoester oligoribonucleotides with 3'-OH and 5'-phosphate ends ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH 7.0: about 50% of maximal activity, pH 10.5: about 60% of maximal activity Homo sapiens