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Literature summary for 3.1.26.5 extracted from

  • Guerrier-Takada, C.; Gardiner, K.; Marsh, T.; Pace, N.; Altman, S.
    The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme (1983), Cell, 35, 849-857.
    View publication on PubMed
Search for more literature for 3.1.26.5

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5-10 mM required Bacillus subtilis
Mg2+ 5-10 mM required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pre-tRNA + H2O Bacillus subtilis
-
 tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA + H2O Escherichia coli
-
 tRNA + 5'-oligoribonucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein the RNA moiety can cleave tRNA precursor molecules in buffer containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine. Under these conditions the protein moiety of the enzymes alone shows no catalytic activity Bacillus subtilis
ribonucleoprotein the RNA moiety can cleave tRNA precursor molecules in buffer containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine. Under these conditions the protein moiety of the enzymes alone shows no catalytic activity Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
RNA moiety Bacillus subtilis
RNA moiety Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5S RNA, precursor to + H2O enzyme complex formed with M1 RNA from E. coli and the protein moiety from E. coli or Bacillus subtilis are active. No activity with the enzyme complex formed with M1 RNA from Bacillus subtilis and the protein from either bacterial species Bacillus subtilis ?
-
 ?
4.5S RNA, precursor to + H2O enzyme complex formed with M1 RNA from E. coli and the protein moiety from E. coli or Bacillus subtilis are active. No activity with the enzyme complex formed with M1 RNA from Bacillus subtilis and the protein from either bacterial species Escherichia coli ?
-
 ?
pre-tRNA + H2O
-
 Bacillus subtilis tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA + H2O
-
 Escherichia coli tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA precursor + H2O RNA moiety can cleave pre-rRNA in buffers containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine Bacillus subtilis tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA precursor + H2O RNA moiety can cleave pre-rRNA in buffers containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine Escherichia coli tRNA + 5'-oligoribonucleotide
-
 ?

Subunits

Subunits Comment Organism
More RNA and protein subunits from one species can complement subunits from the other species in reconstitution experiments Bacillus subtilis
More RNA and protein subunits from one species can complement subunits from the other species in reconstitution experiments Escherichia coli