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Literature summary for 3.1.26.5 extracted from

  • Xiao, S.; Scott, F.; Fierke, C.A.; Engelke, D.R.
    Eukaryotic ribonuclease P: a plurality of ribonucleoprotein enzymes (2002), Annu. Rev. Biochem., 71, 165-189.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme requires low salt levels Bacillus subtilis
additional information enzyme requires very high salt levels Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
additional information complete deletion of the protein subunit is lethal, mutation of P3 loop causes defects in pre-tRNA processing and enzyme RPR1 RNA maturation as well as subunit interaction in vivo Saccharomyces cerevisiae
additional information mutagenesis of the CR-IV region of the enzyme RPR1 RNA results in large reduction of kcat with little effect on Km Bacillus subtilis
additional information mutagenesis of the CR-IV region of the enzyme RPR1 RNA results in large reduction of kcat with little effect on Km Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information substitution of the pro-Rp nonbridging oxygen with sulfur at the scissile bond does not inhibit the enzyme Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Saccharomyces cerevisiae
additional information
-
 additional information kinetics, the rate constant for the scissile bond cleavage is pH-dependent Bacillus subtilis
0.000009
-
 pre-tRNATyr
-
 Saccharomyces cerevisiae
0.000016
-
 pre-tRNA
-
 Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Cyanophora paradoxa 9507
-
chloroplast
-
 Spinacia oleracea 9507
-
chloroplast
-
 Nicotiana tabacum 9507
-
mitochondrion
-
 Cyanophora paradoxa 5739
-
mitochondrion RNases P and MRP Homo sapiens 5739
-
mitochondrion RNases P and MRP, enzyme consists of RNA component Rpm1r and protein component Rpm2p, the latter is required for the processing of the first Saccharomyces cerevisiae 5739
-
nucleolus RNase MRP Homo sapiens 5730
-
nucleolus RNase MRP Saccharomyces cerevisiae 5730
-
nucleus
-
 Cyanophora paradoxa 5634
-
nucleus RNase P Homo sapiens 5634
-
nucleus RNase P Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ preferred metal ion, absolutely required Cyanophora paradoxa
Mg2+ preferred metal ion, absolutely required Bacillus subtilis
Mg2+ preferred metal ion, absolutely required Escherichia coli
Mg2+ preferred metal ion, absolutely required Homo sapiens
Mg2+ preferred metal ion, absolutely required Saccharomyces cerevisiae
Mg2+ preferred metal ion, absolutely required Methanothermobacter thermautotrophicus
additional information divalent metal ions are important cofactors for the catalytic reaction and for substrate binding at the conserved loops CR-II and CR-III in proximity to the substrate aminoacyl stem Escherichia coli
additional information divalent metal ions are important cofactors for the catalytic reaction and for substrate binding at the conserved loops CR-II and CR-III in proximity to the substrate aminoacyl stem, enhancement of substrate affinity by 1000fold Bacillus subtilis
additional information divalent metal ions are important cofactors for the reaction Cyanophora paradoxa
additional information divalent metal ions are important cofactors for the reaction Homo sapiens
additional information divalent metal ions are important cofactors for the reaction Saccharomyces cerevisiae
additional information divalent metal ions are important cofactors for the reaction Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15500
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
15800
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
16300
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
18200
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
19600
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
22500
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
22600
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
32200
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
32900
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
100500
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
112000
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
120000
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens coordination of RNA pathways, overview ?
-
 ?
additional information Saccharomyces cerevisiae coordination of RNA pathways, overview ?
-
 ?
pre-rRNA + H2O Homo sapiens enzyme RNase MRP plays an important role in pre-rRNA processing mature rRNA
-
 ?
pre-rRNA + H2O Saccharomyces cerevisiae enzyme RNase MRP plays an important role in pre-rRNA processing mature rRNA
-
 ?
tRNA precursor + H2O Cyanophora paradoxa essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Bacillus subtilis essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Escherichia coli essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Homo sapiens essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Saccharomyces cerevisiae essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Methanothermobacter thermautotrophicus essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Spinacia oleracea essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Nicotiana tabacum essential enzymes in the biogenesis of tRNA, maturation mature tRNA + 5'-oligonucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Cyanophora paradoxa
-
-
-
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Methanothermobacter thermautotrophicus
-
-
-
Nicotiana tabacum
-
-
-
Saccharomyces cerevisiae
-
-
-
Spinacia oleracea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no ribonucleoprotein
-
 Spinacia oleracea
ribonucleoprotein
-
 Cyanophora paradoxa
ribonucleoprotein
-
 Bacillus subtilis
ribonucleoprotein
-
 Escherichia coli
ribonucleoprotein
-
 Homo sapiens
ribonucleoprotein
-
 Saccharomyces cerevisiae
ribonucleoprotein
-
 Methanothermobacter thermautotrophicus
ribonucleoprotein
-
 Nicotiana tabacum

Purification (Commentary)

Purification (Comment) Organism
-
 Methanothermobacter thermautotrophicus
mitochondrial enzyme, and RNase P partially Homo sapiens
nuclear enzyme Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism Cyanophora paradoxa
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism Homo sapiens
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism Methanothermobacter thermautotrophicus
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism Spinacia oleracea
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism Nicotiana tabacum
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism, CR-IV region is imporatnt for tRNA binding Bacillus subtilis
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism, CR-IV region is important for tRNA binding Escherichia coli
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, tRNA precursor substrate recognition and cleavage mechanism, structure-function relationship of the RNA subunit Saccharomyces cerevisiae
a

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deproteinized pre-rRNA + H2O large number of discrete cleavage sites Saccharomyces cerevisiae mature deproteinized rRNA + 5'-oligonucleotide
-
 ?
additional information coordination of RNA pathways, overview Homo sapiens ?
-
 ?
additional information coordination of RNA pathways, overview Saccharomyces cerevisiae ?
-
 ?
additional information the recombinant holoenzyme and the Rpp20p subunit display ATPase activity Homo sapiens ?
-
 ?
pre-rRNA + H2O enzyme RNase MRP plays an important role in pre-rRNA processing Homo sapiens mature rRNA
-
 ?
pre-rRNA + H2O enzyme RNase MRP plays an important role in pre-rRNA processing Saccharomyces cerevisiae mature rRNA
-
 ?
pre-rRNA + H2O RNase MRP Saccharomyces cerevisiae mature rRNA
-
 ?
pre-rRNA + H2O RNase MRP Homo sapiens mature rRNA + 5'-oligonucleotide
-
 ?
pre-tRNA + H2O
-
 Spinacia oleracea mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Cyanophora paradoxa mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Bacillus subtilis mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Escherichia coli mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Homo sapiens mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Saccharomyces cerevisiae mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Methanothermobacter thermautotrophicus mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Spinacia oleracea mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O essential enzymes in the biogenesis of tRNA, maturation Nicotiana tabacum mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Cyanophora paradoxa mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Bacillus subtilis mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Escherichia coli mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Homo sapiens mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Saccharomyces cerevisiae mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Methanothermobacter thermautotrophicus mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Spinacia oleracea mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide Nicotiana tabacum mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNATyr precursor + H2O cleavage of 5'-terminal oligonucleotide Saccharomyces cerevisiae mature tRNATyr + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?

Subunits

Subunits Comment Organism
decamer 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
More subunit composition, 1 essential RNA subunit Cyanophora paradoxa
More subunit composition, 1 large RNA subunit plus 1 small protein subunit contributing to about 10% of the mass of the holoenzyme Bacillus subtilis
More subunit composition, 1 large RNA subunit plus 1 small protein subunit contributing to about 10% of the mass of the holoenzyme Escherichia coli
More subunit composition, at least 4 protein subunits namely MTH11, MTH687, MTH688, and MTH1618 Methanothermobacter thermautotrophicus
More subunit interactions, subunit composition for RNase P: 1 RNA subunit RPM1 plus at least 1 protein subunit Rpm2p for the mitochondrial ribozyme, 9 protein components for the nuclear enzyme form, namely Pop1p, Pop3p-Pop8p, Rpp1p, and Rpr2p, secondary structure of nuclear enzyme RNA, subunit composition for RNase MRP: 1 RNA subunit NME1 plus 9 protein components for the nuclear enzyme form, namely Pop1p, Pop3p-Pop7p, Pop, Rpp1p, and SNM1, overview, Pop7p is also known as Rpp2p, structure-function relationship of the RNA subunit Saccharomyces cerevisiae
oligomer subunit composition and interaction, 1 essential RNA subunit, i.e. H1 for RNase P or 7-2 for RNase MRP, plus 9 protein components namely Pop1p, Rpp29p, Rpp20p, Rpp30p, Rep38p, Rpp40p, Rpp25p, and Rpp14p for RNase P, or pls 4 protein components namely Pop1p, Rpp29p, Rpp20p, and Rpp30p for RNase MRP, overview Homo sapiens

Synonyms

Synonyms Comment Organism
RNase MRP
-
 Homo sapiens
RNase MRP
-
 Saccharomyces cerevisiae
RNase P
-
 Cyanophora paradoxa
RNase P
-
 Bacillus subtilis
RNase P
-
 Escherichia coli
RNase P
-
 Homo sapiens
RNase P
-
 Saccharomyces cerevisiae
RNase P
-
 Methanothermobacter thermautotrophicus
RNase P
-
 Spinacia oleracea
RNase P
-
 Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 pre-tRNATyr
-
 Saccharomyces cerevisiae
6
-
 tRNA precursor enzyme RNA moiety, pH 7.0 Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae protein subunits Pop1p, Pop3p, Pop4p, Pop6p, Pop7p, Rpp1p, and Rpr2p 4.7 4.6
Saccharomyces cerevisiae protein subunit Pop8p
-
 4.9
Saccharomyces cerevisiae protein subunit Pop5p
-
 6.9