Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme by in vitro transcription, expression of His-tagged enzyme | Bacillus subtilis |
expression of wild-type enzyme by in vitro transcription, expression of His-tagged enzyme | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant enzyme chimeras consisting of either Escherichia coli protein components and Bacillus subtilis RNA component or vice versa | Bacillus subtilis |
additional information | construction of mutant enzyme chimeras consisting of either Escherichia coli protein components and Bacillus subtilis RNA component or vice versa | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bacillus subtilis | |
additional information | - |
additional information | kinetics | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | Bacillus subtilis | - |
mature tRNA + 5'-terminal oligonucleotide | - |
? | |
pre-tRNA + H2O | Escherichia coli | - |
mature tRNA + 5'-terminal oligonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant untagged and His-tagged enzymes | Bacillus subtilis |
recombinant untagged and His-tagged enzymes | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, cleavage of ssRNA, mechanism | Bacillus subtilis | |
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, cleavage of ssRNA, mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate requirements for trans-cleavage of wild-type and mutant hybrid enzymes, overview | Bacillus subtilis | ? | - |
? | |
additional information | substrate requirements for trans-cleavage of wild-type and mutant hybrid enzymes, overview | Escherichia coli | ? | - |
? | |
pre-tRNA + H2O | - |
Bacillus subtilis | mature tRNA + 5'-terminal oligonucleotide | - |
? | |
pre-tRNA + H2O | - |
Escherichia coli | mature tRNA + 5'-terminal oligonucleotide | - |
? | |
pre-tRNA mimic + H2O | small bipartite model substrate, smallest mimic of natural enzyme substrate consisting of a 4 bp stem and a 1 nucleotide 5'-flank, specific trans-cleavage at the canonical enzyme cleavage site | Escherichia coli | ? | - |
? | |
pre-tRNA mimic + H2O | small bipartite model substrates with different numbering of stem and 5'-flank base pairs, specific trans-cleavage at the canonical enzyme cleavage site, overview | Bacillus subtilis | ? | - |
? | |
pre-tRNA mimic a + H2O | mimic of natural enzyme substrate consisting of a 7 bp stem and a 3 nucleotide 5'-flank | Bacillus subtilis | ? | - |
? | |
pre-tRNA mimic a + H2O | mimic of natural enzyme substrate consisting of a 7 bp stem and a 3 nucleotide 5'-flank | Escherichia coli | ? | - |
? | |
pre-tRNA mimic b + H2O | mimic of natural enzyme substrate consisting of a 7 bp stem and a 1 nucleotide 5'-flank | Bacillus subtilis | ? | - |
? | |
pre-tRNA mimic b + H2O | mimic of natural enzyme substrate consisting of a 7 bp stem and a 1 nucleotide 5'-flank | Escherichia coli | ? | - |
? | |
pre-tRNA mimic c + H2O | mimic of natural enzyme substrate consisting of a 4 bp stem and a 3 nucleotide 5'-flank | Bacillus subtilis | ? | - |
? | |
pre-tRNA mimic c + H2O | mimic of natural enzyme substrate consisting of a 4 bp stem and a 3 nucleotide 5'-flank | Escherichia coli | ? | - |
? | |
pre-tRNA mimic d + H2O | mimic of natural enzyme substrate consisting of a 4 bp stem and a 1 nucleotide 5'-flank | Bacillus subtilis | ? | - |
? | |
pre-tRNA mimic d + H2O | mimic of natural enzyme substrate consisting of a 4 bp stem and a 1 nucleotide 5'-flank | Escherichia coli | ? | - |
? | |
ssRNA oligonucleotide + H2O | holoenzyme, specificity with different ssRNA substrates, determination of cleavage sites, overview | Bacillus subtilis | 5'-phospho-3'-hydroxy-ribonucleotides | - |
? | |
ssRNA oligonucleotide + H2O | holoenzyme, specificity with different ssRNA substrates, determination of cleavage sites, overview | Escherichia coli | 5'-phospho-3'-hydroxy-ribonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bacillus subtilis | |
additional information | - |
additional information | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis |
7 | - |
assay at | Escherichia coli |