Inhibitors | Comment | Organism | Structure |
---|---|---|---|
neomycin B | the inhibition is of non-competitive type, increasing Mg2+ concentrations from 3 to 20 mM, at a constant neomycin concentration (0.05 mM), result in a considerable recovery of the activity (about 40%) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000244 | - |
SupS1 precursor | in 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mM MgCl2, 2.5 mM EGTA and 0.5 U Rnasin | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, the optimum is at 5 mM MgCl2, when reactions are carried out at pH 7.5 and 37°C | Homo sapiens | |
NH4+ | dependent on, the optimum is at 70 mM NH4Cl, when reactions are carried out at pH 7.5 and 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phosphocellulose column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mm MgCl2, 2.5 mM EGTA, and 0.5 U RNasin, few weeks, the enzyme activity remains stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | RNase P is a key enzyme acting early in the tRNA biogenesis pathway, which catalyses the endonucleolytic cleavage of the 5' leader sequence of precursor tRNAs and generates their 5' mature end | Homo sapiens | tRNA + 5'-oligoribonucleotide | - |
? | |
SupS1 precursor + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
neomycin B | in 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mM MgCl2, 2.5 mM EGTA and 0.5 U Rnasin | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.074 | - |
in 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mM MgCl2, 2.5 mM EGTA and 0.5 U Rnasin | Homo sapiens | neomycin B |