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Literature summary for 3.1.26.5 extracted from

  • Hara, T.; Terada, A.; Yamaguchi, H.; Nakashima, T.; Kakuta, Y.; Kimura, M.
    The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNA from Pyrococcus horikoshii OT3 (2011), Biosci. Biotechnol. Biochem., 75, 816-819.
    View publication on PubMed
Search for more literature for 3.1.26.5

Protein Variants

Protein Variants Comment Organism
additional information generation of several enzyme mutants with deleted stem loops in their RNA part. The mutants show reduced activity Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of deletion mutants Pyrococcus horikoshii
0.00000009
-
 pre-tRNATyr wild-type enzyme, pH not specified in the publication, 65°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-
Pyrococcus horikoshii OT-3
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNATyr + H2O activity assay using in vitro reconstituted particles Pyrococcus horikoshii tRNATyr + 5' leader of tRNA
-
 ?
pre-tRNATyr + H2O activity assay using in vitro reconstituted particles Pyrococcus horikoshii OT-3 tRNATyr + 5' leader of tRNA
-
 ?

Synonyms

Synonyms Comment Organism
PhoPRNA
-
 Pyrococcus horikoshii
RNase P RNA
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 the deletion mutants show a slightly reduced optimal temperature compared to the wild-type enzyme Pyrococcus horikoshii
65 70 wild-type enzyme Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 pre-tRNATyr wild-type enzyme, pH not specified in the publication, 65°C Pyrococcus horikoshii

General Information

General Information Comment Organism
physiological function the stem loops of the RNase P RNA are required as binding sites for the proteins, their interactions are predominantly involved in stabilizing the active conformation of the enzyme Pyrococcus horikoshii