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Literature summary for 3.1.26.5 extracted from
- Purification and characterization of potato ribonuclease P (2013), J. Plant Biochem. Biotechnol., 22, 425-433.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis of two cDNAs encoding two putative protein subunits of potato ribonuclease P, StPop5 and StRpp25, subcloning in Escherichia coli strain M15, recombinant overexpression as His6-tagged proteins in Escherichia coli strain BL21(DE3) | Solanum tuberosum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Solanum tuberosum | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Solanum tuberosum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
x * 18000, recombinant His6-tagged protein StPop5, SDS-PAGE, x * 33000, recombinant His6-tagged protein StRpp25, SDS-PAGE | Solanum tuberosum |
| 33000 | - |
x * 18000, recombinant His6-tagged protein StPop5, SDS-PAGE, x * 33000, recombinant His6-tagged protein StRpp25, SDS-PAGE | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Solanum tuberosum | the natural substrate is precursor tRNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| ribonucleoprotein | - |
Solanum tuberosum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native nuclear enzyme partially purified from leaves employing anion exchange chromatography, and from floral buds employing two different steps of anion exchange chromatography, ammonium sulfate fractionation, and gel filtration, purification of recombinant His6-tagged proteins StPop5 and StRpp25 from Escherichia coli strain BL21(DE3) | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | - |
Solanum tuberosum | - |
| leaf | - |
Solanum tuberosum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the natural substrate is precursor tRNA | Solanum tuberosum | ? | - |
? | |
| additional information | assay substrate is tobacco chloroplast precursor tRNAGly | Solanum tuberosum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 18000, recombinant His6-tagged protein StPop5, SDS-PAGE, x * 33000, recombinant His6-tagged protein StRpp25, SDS-PAGE | Solanum tuberosum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase P | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Solanum tuberosum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the two protein subunits StPop5 and StRpp25 are associated with with floral bud enzyme activity but not with leaf enzyme activity | Solanum tuberosum |
| physiological function | the ribonucleoprotein endoribonuclease is responsible for 5' maturation of precursor tRNA | Solanum tuberosum |
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