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Literature summary for 3.1.26.5 extracted from

  • Lai, S.M.; Lai, L.B.; Foster, M.P.; Gopalan, V.
    The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA (2014), Nucleic Acids Res., 42, 13328-13338.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Protein Variants

Protein Variants Comment Organism
C71V the single-Cys substitutions are introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein. The C71V parental reference is more active than the wild type enzyme Pyrococcus furiosus
K42C mutation does not affect activity. The single-Cys substitution is introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein Pyrococcus furiosus
R46C mutation results in 28% decrease in activity. The single-Cys substitution is introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein Pyrococcus furiosus
V95C mutation results in 6% decrease in activity. The single-Cys substitution is introduced into a Cys-less Pfu L7Ae template C71V (i.e. RNA-binding protein L7Ae, subunit of archaeal RNase P). The native C71, which is partly buried, is mutated to Val to preserve the native fold and hydrophobic core of the protein Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNATyr + H2O pre-tRNATyr from Escherichia coli Pyrococcus furiosus tRNATyr + 5'-oligoribonucleotide
-
 ?

Subunits

Subunits Comment Organism
oligomer the RNA-binding protein L7Ae (UniProt: Q8U160) is a subunit of the archaeal RNase P ribonucleoprotein complex. The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
RNase P
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 Pyrococcus furiosus
RPR
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 Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pyrococcus furiosus