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Literature summary for 3.1.26.5 extracted from

  • Jiang, X.; Chen, Y.C.; Gong, H.; Trang, P.; Lu, S.; Liu, F.
    Ribonuclease P-mediated inhibition of human cytomegalovirus gene expression and replication induced by engineered external guide sequences (2012), RNA Biol., 9, 1186-1195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant external guide sequences in brief, amphotropic PA317 cells Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the natural substrate is precursor tRNA ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the natural substrate is precursor tRNA Homo sapiens ?
-
?
additional information constructed substrate: a hybridized complex of an external guide sequence and a target RNA, e.g. mRNA, that resembles the structure of a tRNA, structure overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
RNase P
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme RNase P is a tRNA processing enzyme. The enzyme can mediate inhibition of human cytomegalovirus gene expression and replication in U373MG cells, and the viral capsid formation, induced by engineered external guide sequences, overview. External guide sequences (EGSs) are RNA molecules that can bind to a target mRNA and direct ribonuclease P for specific cleavage of the target mRNA. Construction of EGS variants that efficiently direct human RNase P to cleave a target mRNA, coding for human cytomegalovirus capsid scaffolding protein and assemblin, in vitro. The EGS variant is about 40fold more active in directing human enzyme to cleave the mRNA in vitro than the EGS derived from a natural tRNA Homo sapiens