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Literature summary for 3.1.26.5 extracted from
- Characterization of RNase P from Thermotoga maritima (2001), Nucleic Acids Res., 29, 880-885 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the protein subunit in Escherichia coli. The cloned protein is reconstituted with the RNA subunit transcribed in vitro | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000223 | - |
tRNATyr precursor | pH 7, 50°C | Thermotoga maritima |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal concentration: 200 mM | Thermotoga maritima |  |
| MgCl2 | optimal activity at 5 mM | Thermotoga maritima | |
| NaCl | optimal concentration: 50 mM | Thermotoga maritima | |
| NH4Cl | optimal concentration: 200-400 mM | Thermotoga maritima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
| Thermotoga maritima DSM 3109 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNATyr precursor + H2O | - |
Thermotoga maritima | mature tRNATyr + 5'-terminal RNA oligonucleotide | - |
? | |
| tRNATyr precursor + H2O | - |
Thermotoga maritima DSM 3109 | mature tRNATyr + 5'-terminal RNA oligonucleotide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase P | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 55 | 40°C: about 45% of maximal activity, 55°C: about 25% of maximal activity | Thermotoga maritima |
| 65 | - |
no activity at 65°C or above | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.137 | - |
tRNATyr precursor | pH 7, 50°C | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Thermotoga maritima |
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Release 2023.1 (January 2023)
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