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Literature summary for 3.1.26.5 extracted from

  • Singh, A.; Ramteke, A.K.; Afroz, T.; Batra, J.K.
    Insight into the role of histidine in RNR motif of protein component of RNase P of M. tuberculosis in catalysis (2016), IUBMB Life, 68, 178-189 .
    View publication on PubMed
Search for more literature for 3.1.26.5

Cloned(Commentary)

Cloned (Comment) Organism
expression of the protein and RNA components of mycobacterial RNase P in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H67A spectrum of mutant is moderately altered at 37°C, indicative of subtle structural changes arising due to the mutation. The H67A protein shows significant loss in secondary structure at 45°C, which further enhances at 55°C. The loss of structure with increase in temperature is relatively less in the case of wild-type and H67N proteins. kcat/Km-value is 1.9fold lower than the value for the wild-type enzyme Mycobacterium tuberculosis
H67N spectrum of mutant is moderately altered at 37°C, indicative of subtle structural changes arising due to the mutation. The H67A protein shows significant loss in secondary structure at 45°C, which further enhances at 55°C. The loss of structure with increase in temperature is relatively less in the case of wild-type and H67N proteins. kcat/Km-value is 1.4fold lower than the value for the wild-type enzyme Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the RNA component alone shows activity on pre-tRNAala substrate at high magnesium concentrations (50 mM). The RNA and protein components associate together to manifest catalytic activity at low magnesium concentrations (20 mM) Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WGZ3 the enzyme consists of a catalytic RNA component (M1 or rnpB) and a protein subunit rnpA (P9WGZ3)
-
Mycobacterium tuberculosis H37Rv P9WGZ3 the enzyme consists of a catalytic RNA component (M1 or rnpB) and a protein subunit rnpA (P9WGZ3)
-

Purification (Commentary)

Purification (Comment) Organism
purification of the recombinant protein and RNA components and reconstitution of the holoenzyme in vitro Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA-Ala + H2O the RNA component alone shows activity on pre-tRNAala substrate at high magnesium concentrations (50 mM). The RNA and protein components associate together to manifest catalytic activity at low magnesium concentrations (20 mM). The histidine 67 in the RNR motif of RNase P protein component is important for the catalytic activity and stability of the enzyme Mycobacterium tuberculosis mature tRNA-Ala + 5'-terminal oligonucleotide
-
 ?
pre-tRNA-Ala + H2O the RNA component alone shows activity on pre-tRNAala substrate at high magnesium concentrations (50 mM). The RNA and protein components associate together to manifest catalytic activity at low magnesium concentrations (20 mM). The histidine 67 in the RNR motif of RNase P protein component is important for the catalytic activity and stability of the enzyme Mycobacterium tuberculosis H37Rv mature tRNA-Ala + 5'-terminal oligonucleotide
-
 ?

Subunits

Subunits Comment Organism
dimer all bacterial RNase Ps have one RNA and one protein component. A conserved RNR motif in bacterial RNase P protein components is involved in their interaction with the RNA component Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
RNase P
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: about 90% of maximal activity, 45°C: about 65% of maximal activity Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function the enzyme is involved in the 5' end processing of pre-tRNAs Mycobacterium tuberculosis