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Literature summary for 3.1.26.5 extracted from

  • Howard, M.J.; Klemm, B.P.; Fierke, C.A.
    Mechanistic Studies Reveal Similar Catalytic Strategies for Phosphodiester Bond Hydrolysis by Protein-only and RNA-dependent Ribonuclease P (2015), J. Biol. Chem., 290, 13454-13464 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Protein Variants

Protein Variants Comment Organism
D399A mutation significantly reduces activity. No product formation is observed after a 30-min incubation under standard STO assay conditions (1 mM MgCl2). No increase in activity, when Mg2+ concentration increases from 1 mM to 20 mM Arabidopsis thaliana
D474A mutation significantly reduces activity. No product formation is observed after a 30-min incubation under standard STO assay conditions (1 mM MgCl2). Activity increases significantly at the 20 mM Mg2+ compared to 1 mM Mg2+ Arabidopsis thaliana
D475A mutation significantly reduces activity. No product formation is observed after a 30-min incubation under standard STO assay conditions (1 mM MgCl2). Activity increases significantly at the 20 mM Mg2+ compared to 1 mM Mg2+ Arabidopsis thaliana
D493A mutation significantly reduces activity. No product formation is observed after a 30-min incubation under standard STO assay conditions (1 mM MgCl2). No increase in activity, when Mg2+ concentration increases from 1 mM to 20 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 pre-tRNA-Asp 25°C, pH 7.8 Arabidopsis thaliana
0.00063
-
 pre-tRNA-Cys 25°C, pH 7.8 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Arabidopsis thaliana
Mg2+ activates, the enzyme requires at least two Mg2+ ions for optimal catalysis. Mg2+ ions bind cooperatively to PRORP1 Arabidopsis thaliana
Mn2+ activates, two manganese ions are bound to the active site interacting with four conserved aspartate residues (D399A, D474A, D475A, and D493A) through both inner and outer sphere interactions Arabidopsis thaliana
Ni2+ activates Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q66GI4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA-Asp + H2O pre-tRNA-Asp from Bacillus subtilis Arabidopsis thaliana mature tRNA-Asp + 5'-oligonucleotide
-
 ?
pre-tRNA-Cys + H2O pre-tRNA-Cys from Arabidopsis thaliana Arabidopsis thaliana mature tRNA-Cys + 5'-oligonucleotide
-
 ?

Synonyms

Synonyms Comment Organism
PRORP1
-
 Arabidopsis thaliana
protein-only ribonuclease P
-
 Arabidopsis thaliana
ribonuclease P
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 pre-tRNA-Asp 25°C, pH 7.8 Arabidopsis thaliana
0.06
-
 pre-tRNA-Cys 25°C, pH 7.8 Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 pre-tRNA-Asp 25°C, pH 7.8 Arabidopsis thaliana
98
-
 pre-tRNA-Cys 25°C, pH 7.8 Arabidopsis thaliana