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Literature summary for 3.1.3.1 extracted from

  • Bhatti, A.R.; Alvi, A.; Chaudhry, G.R.
    Evidence on the presence of two distinct alkaline phosphatases in Serratia marcescens (2000), FEMS Microbiol. Lett., 182, 131-135.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl 1 M, activation of enzyme form IAPase Serratia marcescens

General Stability

General Stability Organism
Mg2+ protects against thermal inactivation Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
NaCl 1 M, inhibition of enzyme form CAPase Serratia marcescens
phosphate competitive inhibition of both enzyme forms Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
4-nitrophenyl phosphate IAPase Serratia marcescens
0.057
-
4-nitrophenyl phosphate CAPase Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase
-
Serratia marcescens 211
-
2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase
-

Purification (Commentary)

Purification (Comment) Organism
2 distinct enzyme forms, CAPase 212fold and 4 isozymes of IAPase 5-87fold Serratia marcescens

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
purified isozyme IV of IAPase Serratia marcescens
22.2
-
purified isozyme III of IAPase Serratia marcescens
30.8
-
purified isozyme I of IAPase Serratia marcescens
43.5
-
purified isozyme II of IAPase Serratia marcescens
106
-
purified CAPase Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Serratia marcescens 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Serratia marcescens 211 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Serratia marcescens
APase
-
Serratia marcescens
CAPase
-
Serratia marcescens
IAPase
-
Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Mg2+ protects against thermal inactivation up to 80°C Serratia marcescens
95
-
5 min, in absence or presence of 10 mM Mg2+, inactivation of CAPase, while IApase is relatively stable showing loss of 30% activity after 5 min and loss of 80% activity after 30 min Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 10 IAPase Serratia marcescens
9.5
-
CAPase Serratia marcescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0053
-
phosphate IAPase Serratia marcescens
0.76
-
phosphate CAPase Serratia marcescens