Any feedback?
Literature summary for 3.1.3.1 extracted from
- Characterization of a monomeric Escherichia coli alkaline phosphatase formed upon a single amino acid substitution (2003), J. Biol. Chem., 278, 23497-23501.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Zn2+ and Mg2+ do not affect the mutant T59R enzyme | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the wild-type and mutant enzymes | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T59A | site-directed mutagenesis, exists as a dimer, shows catalytic activity and metal content similar to the wild-type enzyme | Escherichia coli |
| T59R | site-directed mutagenesis, exists as a monomer, shows 10000fold reduced activity compared to the wild-type, highly reduced metal content, highly reduced thermal stability | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Zn2+ and Mg2+ do not affect the mutant T59R enzyme | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0218 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Escherichia coli |  |
| 0.0249 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59A | Escherichia coli | |
| 0.0392 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59R | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | metalloenzyme, mutant T59A contains sn amount similar to the wild-type enzyme, while mutant T59R has almost undetectable amounts of bound metal | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
recombinant mutant T59R, sucrose density gradient sedimentation | Escherichia coli |
| 87000 | - |
recombinant wild-type and mutant T59A enzymes, sucrose density gradient sedimentation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P00634 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Escherichia coli | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 44000, SDS-PAGE, recombinant wild-type and mutant T59A | Escherichia coli |
| monomer | 1 * 44000, SDS-PAGE, recombinant mutant T59R | Escherichia coli |
| More | Thr59 is involved in stabilization of the dimeric interface, structural analysis | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phosphatase | - |
Escherichia coli |
| AP | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00021 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59R | Escherichia coli | |
| 1.5 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59A | Escherichia coli | |
| 2.3 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Escherichia coli | |
| 56.3 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59A | Escherichia coli | |
| 74.7 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
