General Stability | Organism |
---|---|
requires Mg2+ and dithiothreitol for stability throughout its purification | Onchocerca ochengi |
sodium dodecyl sulphate at 2% (w/v) stabilises the enzyme during freezing | Onchocerca ochengi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HPO42- | competitive | Onchocerca ochengi | |
L-Phe | mixed-type inhibition | Onchocerca ochengi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
4-nitrophenyl phosphate | 37°C, pH 9.8 | Onchocerca ochengi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Onchocerca ochengi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Onchocerca ochengi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.86 | - |
- |
Onchocerca ochengi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Onchocerca ochengi | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the 90000 Da monomeric species dimerises to about 180000 Da upon purification | Onchocerca ochengi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.2 | - |
- |
Onchocerca ochengi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11 | pH 9.0: about 35% of maximal activity, pH 11.0: about 35% of maximal activity | Onchocerca ochengi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.18 | - |
L-Phe | 37°C, pH 9.8 | Onchocerca ochengi | |
3.33 | - |
HPO42- | 37°C, pH 9.8 | Onchocerca ochengi |