Literature summary for 3.1.3.1 extracted from

Zhang, Y.; Ji, C.; Zhang, X.; Yang, Z.; Peng, J.; Qiu, R.; Xie, Y.; Mao, Y.
A moderately thermostable alkaline phosphatase from Geobacillus thermodenitrificans T2: cloning, expression and biochemical characterization (2008), Appl. Biochem. Biotechnol., 151, 81-92.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli and purified as a hexa-His-tagged fusion protein	Geobacillus thermodenitrificans

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	10 mM, 99% inhibition	Geobacillus thermodenitrificans
inorganic phosphate	-	Geobacillus thermodenitrificans
additional information	enzyme is resistant to urea and dithioreitol	Geobacillus thermodenitrificans
vanadate	10 mM, 89% inhibition	Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0315	-	4-nitrophenyl phosphate	-	Geobacillus thermodenitrificans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	a divalent cation is essential, with a combination of Mg2+ and Co2+ or Zn2+ preferred	Geobacillus thermodenitrificans
Mg2+	a divalent cation is essential, with a combination of Mg2+ and Co2+ or Zn2+ preferred	Geobacillus thermodenitrificans
Zn2+	a divalent cation is essential, with a combination of Mg2+ and Co2+ or Zn2+ preferred	Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42150	-	deduced from cDNA	Geobacillus thermodenitrificans

Organism

Organism	UniProt	Comment	Textmining
Geobacillus thermodenitrificans	A8WEG4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Geobacillus thermodenitrificans	4-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
alkaline phosphatase	-	Geobacillus thermodenitrificans
Gtd AP	-	Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	optimal temperature	Geobacillus thermodenitrificans

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
45	60	enzyme retains a high activity between 45°C-60°C	Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	enzyme gets quickly inactivated at 80°C for 15 min, exhibiting a half-life of 8 min at 70°C	Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Geobacillus thermodenitrificans

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Release 2023.1 (January 2023)