Application | Comment | Organism |
---|---|---|
medicine | this particular V406A mutation highlights the structural importance of the crown domain with respect to the catalytic function of TNSALP | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in transiently transfected COS-1 cells and in stable CHO-K1 Tet-On cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V406A | mutant protein shows a markedly reduced alkaline phosphatase activity. Mutant is conveyed to the Golgi apparatus and incorporated into a cold detergent insoluble fraction (raft) at a rate similar to that of the wild-type. Mutant shows increased susceptibility to digestion by trypsin or proteinase K. When purified as a glycosylphosphatidylinositol-anchorless soluble form, mutant protein exhibits a remarkably lower Kcat /Km value compared with that of the wild-type TNSALP | Homo sapiens |
V406F | replacement with phenylalanine results in a low enzyme activity, even though TNSALP (V406F) is processed to the 80 kDa mature form similarly to TNSALP (V406A) and appears on the cell surface like the wild-type protein | Homo sapiens |
V406I | similar to wild-type, leucine and isoleucine can be successfully substituted for the valine residue | Homo sapiens |
V406L | similar to wild-type, leucine and isoleucine can be successfully substituted for the valine residue | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
4-nitrophenyl phosphate | pH 10.5, mutant V406A | Homo sapiens | |
0.21 | - |
4-nitrophenyl phosphate | pH 10.5, wild-type | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
immunoblotting, immature form bearing high mannose-type N-linked oligosaccharides | Homo sapiens |
80000 | - |
immunoblotting, mature form bearing complex-type oligosaccharides | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | sucrose density gradient centrifugation, wild-type and V406A form a homodimer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
tissue-nonspecific alkaline phosphatase | - |
Homo sapiens |
TNSALP | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
4-nitrophenyl phosphate | pH 10.5, mutant V406A | Homo sapiens | |
971 | - |
4-nitrophenyl phosphate | pH 10.5, wild-type | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
assay at | Homo sapiens |