Application | Comment | Organism |
---|---|---|
analysis | construction o a fusion proein between the ZZ protein, two specific Fc-fragment-binding domains derived from the B-domain of SpA, and alkaline phosphatase. The ZZ-AP fusion retains full parental activities and exhibits an approximately tenfold higher sensitivity than that of ZZ-AP conjugate in enzyme-linked immunosorbent assay. The ZZ-AP fusion is a promising immunoreagent for IgG detection and a potential biolinker between antibodies and reporter enzymes. Compared with the parents, the equilibrium dissociation constant of ZZ-AP conjugate is decreased by 32% and catalytic activity is decreased by 24% | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
4-nitrophenyl phosphate | fusion protein with ZZ protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.29 | - |
4-nitrophenyl phosphate | native enzyme, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.31 | - |
4-nitrophenyl phosphate | conjugate between enzyme and ZZ protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Escherichia coli | 4-nitrophenol + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
285 | - |
4-nitrophenyl phosphate | conjugate between enzyme and ZZ protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
370 | - |
4-nitrophenyl phosphate | fusion protein with ZZ protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
378 | - |
4-nitrophenyl phosphate | native enzyme, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |