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Literature summary for 3.1.3.1 extracted from

  • Golotin, V.; Balabanova, L.; Likhatskaya, G.; Rasskazov, V.
    Recombinant production and characterization of a highly active alkaline phosphatase from marine bacterium Cobetia marina (2015), Mar. Biotechnol., 17, 130-143.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cobetia marina

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: EDTA Cobetia marina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
4-nitrophenyl phosphate 25°C, pH 18.0, Tris-HCl buffer Cobetia marina
0.3
-
4-nitrophenyl phosphate 25°C, pH 18.0, diethanolamine buffer Cobetia marina
2.9
-
4-nitrophenyl phosphate 25°C, pH 10.3, Tris-HCl buffer Cobetia marina
13.2
-
4-nitrophenyl phosphate 25°C, pH 10.3, diethanolamine buffer Cobetia marina

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 0.1-0.2 M of NaCl or KCl, 2fold activation Cobetia marina
additional information enzyme does not require the presence of exogenous divalent cations Cobetia marina
NaCl 0.1-0.2 M of NaCl or KCl, 2fold activation Cobetia marina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54370
-
x * 54370, calculated Cobetia marina

Organism

Organism UniProt Comment Textmining
Cobetia marina Q1W622
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3800
-
pH 9.0, 37°C Cobetia marina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Cobetia marina 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 54370, calculated Cobetia marina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
Cobetia marina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
half-life 27 min, presence of 2 mM EDTA Cobetia marina
45
-
half-life 16 min, presence of 2 mM EDTA Cobetia marina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1500
-
4-nitrophenyl phosphate 25°C, pH 18.0, Tris-HCl buffer Cobetia marina
3710
-
4-nitrophenyl phosphate 25°C, pH 10.3, Tris-HCl buffer Cobetia marina
24000
-
4-nitrophenyl phosphate 25°C, pH 18.0, diethanolamine buffer Cobetia marina
28300
-
4-nitrophenyl phosphate 25°C, pH 10.3, diethanolamine buffer Cobetia marina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10.3
-
Cobetia marina

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11
-
Cobetia marina

pI Value

Organism Comment pI Value Maximum pI Value
Cobetia marina calculated
-
4.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1200
-
4-nitrophenyl phosphate 25°C, pH 10.3, Tris-HCl buffer Cobetia marina
2100
-
4-nitrophenyl phosphate 25°C, pH 10.3, diethanolamine buffer Cobetia marina
33000
-
4-nitrophenyl phosphate 25°C, pH 18.0, Tris-HCl buffer Cobetia marina
82000
-
4-nitrophenyl phosphate 25°C, pH 18.0, diethanolamine buffer Cobetia marina