Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cobetia marina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA | Cobetia marina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
4-nitrophenyl phosphate | 25°C, pH 18.0, Tris-HCl buffer | Cobetia marina | |
0.3 | - |
4-nitrophenyl phosphate | 25°C, pH 18.0, diethanolamine buffer | Cobetia marina | |
2.9 | - |
4-nitrophenyl phosphate | 25°C, pH 10.3, Tris-HCl buffer | Cobetia marina | |
13.2 | - |
4-nitrophenyl phosphate | 25°C, pH 10.3, diethanolamine buffer | Cobetia marina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 0.1-0.2 M of NaCl or KCl, 2fold activation | Cobetia marina | |
additional information | enzyme does not require the presence of exogenous divalent cations | Cobetia marina | |
NaCl | 0.1-0.2 M of NaCl or KCl, 2fold activation | Cobetia marina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54370 | - |
x * 54370, calculated | Cobetia marina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cobetia marina | Q1W622 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3800 | - |
pH 9.0, 37°C | Cobetia marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Cobetia marina | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54370, calculated | Cobetia marina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Cobetia marina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life 27 min, presence of 2 mM EDTA | Cobetia marina |
45 | - |
half-life 16 min, presence of 2 mM EDTA | Cobetia marina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1500 | - |
4-nitrophenyl phosphate | 25°C, pH 18.0, Tris-HCl buffer | Cobetia marina | |
3710 | - |
4-nitrophenyl phosphate | 25°C, pH 10.3, Tris-HCl buffer | Cobetia marina | |
24000 | - |
4-nitrophenyl phosphate | 25°C, pH 18.0, diethanolamine buffer | Cobetia marina | |
28300 | - |
4-nitrophenyl phosphate | 25°C, pH 10.3, diethanolamine buffer | Cobetia marina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | 10.3 | - |
Cobetia marina |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Cobetia marina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cobetia marina | calculated | - |
4.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1200 | - |
4-nitrophenyl phosphate | 25°C, pH 10.3, Tris-HCl buffer | Cobetia marina | |
2100 | - |
4-nitrophenyl phosphate | 25°C, pH 10.3, diethanolamine buffer | Cobetia marina | |
33000 | - |
4-nitrophenyl phosphate | 25°C, pH 18.0, Tris-HCl buffer | Cobetia marina | |
82000 | - |
4-nitrophenyl phosphate | 25°C, pH 18.0, diethanolamine buffer | Cobetia marina |