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Literature summary for 3.1.3.1 extracted from

  • Darwish, D.; Ibrahim, M.; Ghazy, A.
    Purification and characterization of alkaline phosphatase from water buffalo liver (Bubalus bubalis) (2016), Res. J. Pharm. Biol. Chem. Sci., 7, 1869-1880 .
No PubMed abstract available
Search for more literature for 3.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol the P2 isoenzyme is more sensitive to 2-mercaptoethanol than P1 Bubalus bubalis
cysteine
-
 Bubalus bubalis
dithiothreitol
-
 Bubalus bubalis
EDTA
-
 Bubalus bubalis
GSH
-
 Bubalus bubalis
L-phenylalanine the P2 isoenzyme is more sensitive to L-phenylalanine than P1 Bubalus bubalis
Zn2+ potent inhibitor Bubalus bubalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Bubalus bubalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
188000
-
 isoenzyme P1, gel filtration Bubalus bubalis
194000
-
 isoenzyme P2, gel filtration Bubalus bubalis

Organism

Organism UniProt Comment Textmining
Bubalus bubalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Sephacryl S-300 gel filtration Bubalus bubalis

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bubalus bubalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Bubalus bubalis 4-nitrophenol + phosphate
-
 ?
alpha-naphthyl phosphate + H2O
-
 Bubalus bubalis alpha-naphthol + phosphate
-
 ?

Subunits

Subunits Comment Organism
homotrimer
-
 Bubalus bubalis

Synonyms

Synonyms Comment Organism
ALPase
-
 Bubalus bubalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Bubalus bubalis

pI Value

Organism Comment pI Value Maximum pI Value
Bubalus bubalis isoelectric focusing 5.8 5.6