Any feedback?
Literature summary for 3.1.3.1 extracted from
- Some properties of the alkaline phosphatase of HeLa cells (1972), Enzymologia, 42, 329-341.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Be2+ | - |
Homo sapiens |  |
| Phe | - |
Homo sapiens | |
| Zn2+ | above 0.02 mM | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 64 | - |
120 min, about 15% loss of activity of the cytolasmic isoenzyme, about 5% loss of activity of the nuclear isoenzyme | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
hydrolysis of 2 mM p-nitrophenyl phosphate, borate buffer | Homo sapiens |
| 9.8 | - |
hydrolysis of 2 mM p-nitrophenyl phosphate, 2-amino-2-methylpropan-1-ol hydrochloride buffer | Homo sapiens |
| 10.5 | - |
hydrolysis of 8 mM p-nitrophenyl phosphate, 2-amino-2-methylpropan-1-ol hydrochloride buffer | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
