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Literature summary for 3.1.3.1 extracted from

  • Chandran Nair, B.; Majeska, R.J.; Rodan, G.A.
    Rat alkaline phosphatase. I. Purification and characterization of the enzyme from osteosarcoma: generation of monoclonal and polyclonal antibodies (1987), Arch. Biochem. Biophys., 254, 18-27.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
L-homoarginine powerful inhibitor of the bone/liver/kidney isoenzyme and for the osteosarcoma cell enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 p-nitrophenyl phosphate
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 x * 82000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
 Rattus norvegicus p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
 hydrolysis of p-nitrophenyl phosphate Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
9.2 11 pH 9.2: about 40% of maximal activity, pH 11.0: about 60% of maximal activity Rattus norvegicus