Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phe | 20 mM, 82-99% inhibition of the soluble enzyme form, 68-95% inhibition of the particulate enzyme form | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
beta-Glycerophosphate | soluble enzyme form | Rattus norvegicus | |
0.38 | - |
beta-Glycerophosphate | membranous enzyme form | Rattus norvegicus | |
0.39 | - |
ATP | membranous enzyme form | Rattus norvegicus | |
0.44 | - |
p-nitrophenyl phosphate | membranous enzyme form | Rattus norvegicus | |
0.45 | - |
p-nitrophenyl phosphate | soluble enzyme form | Rattus norvegicus | |
0.48 | - |
ATP | soluble enzyme form | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, soluble enzyme form, SDS-PAGE | Rattus norvegicus |
56000 | - |
2 * 56000, membranous enzyme form, SDS-PAGE | Rattus norvegicus |
95000 | - |
soluble enzyme form, gel filtration | Rattus norvegicus |
98000 | - |
membranous enzyme form, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | glycoprotein, the soluble form contains more fucose and N-acetylgalactosamine than does the membranous form. The soluble enzyme form contains 19.8% carbohydrate, the membranous enzyme form contains 16% carbohydrate | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | the soluble and the membranous form are closely related but distinct isoenzymes | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
147 | - |
membranous isoenzyme | Rattus norvegicus |
341 | - |
soluble isoenzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phosphoglycerate + H2O | - |
Rattus norvegicus | glycerol + phosphate | - |
? | |
ATP + H2O | - |
Rattus norvegicus | ? | - |
? | |
beta-glycerophosphate + H2O | - |
Rattus norvegicus | glycerol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, membranous enzyme form, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 47000, soluble enzyme form, SDS-PAGE | Rattus norvegicus |