Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 10 mM, 127% of initial activity, 50 mM, no residual activity | Malus domestica | |
additional information | 60 min at 60°C, 85% increase in enzyme activity | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 10 mM, 127% of initial activity, 50 mM, no residual activity | Malus domestica | |
AMP | non-competitive. AMP, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate interact in a synergistic way to inhibit the enzyme activity | Malus domestica | |
Ca2+ | 0.05 mM, 41% residual activity | Malus domestica | |
Cu2+ | 1 mM, no residual activity | Malus domestica | |
D-fructose 1,6-bisphosphate | competitive, changes substrate saturation curve from hyperbolic to sigmoidal. AMP, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate interact in a synergistic way to inhibit the enzyme activity | Malus domestica | |
D-fructose 6-phosphate | mixed-type. AMP, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate interact in a synergistic way to inhibit the enzyme activity | Malus domestica | |
Hg2+ | 0.1 mM, no residual activity | Malus domestica | |
Li+ | - |
Malus domestica | |
additional information | sorbitol increases the susceptibility of the enzyme to inhibition by high concentrations of D-fructose 1,6-bisphosphate | Malus domestica | |
Zn2+ | - |
Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
D-fructose 1,6-bisphosphate | - |
Malus domestica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Malus domestica | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | or Mn2+, required. Km-value for Mg2+ is 0.59 mM | Malus domestica | |
Mn2+ | or Mg2+, required. Km-value for Mn2+ is 0.062 mM. Mn2+ enhances Mg2+ activated enzyme activity | Malus domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
4 * 37000, SDS-PAGE | Malus domestica |
150000 | - |
gel filtration | Malus domestica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | enzyme is highly specific for D-fructose 1,6-bisphosphate. At 1 mM substrate, activity is only 40% of the activity at 0.06 mM substrate | Malus domestica | D-fructose 6-phosphate + phosphate | - |
? | |
additional information | no substrate: D-glucose 1-phosphate, D-glucose 6-phosphate, sorbitol 6-phosphate, phosphoenolpyruvate, D-fructose 6-phosphate, 3-glycerate phosphate, dihydroxyacetone | Malus domestica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37000, SDS-PAGE | Malus domestica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
purification at 4°C, loss of 80% of the enzyme activity | Malus domestica |
60 | - |
60 min, 85% increase in enzyme activity | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.8 | at 5 mM Mg2+ | Malus domestica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
D-fructose 6-phosphate | - |
Malus domestica |