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Literature summary for 3.1.3.16 extracted from
- Chicken anemia virus VP2 is a novel dual specificity protein phosphatase (2002), J. Biol. Chem., 277, 39566-39573.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as a GST-fusion protein in Escherichia coli | chicken anemia virus |
| expression as a GST-fusion protein in Escherichia coli | TTV-like mini virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C95S | loss in activity | chicken anemia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaF | specific inhibition of activity for the RRApTVA substrate by 50 mM | chicken anemia virus |  |
| NaF | specific inhibition of activity for the RRApTVA substrate by 50 mM | TTV-like mini virus | |
| orthovanadate | specific inhibition of activity for the ENDpYINASL substrate by 0.01 mM | chicken anemia virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0189 | - |
ENDpYINASL | 25°C, pH 7 | chicken anemia virus | |
| 0.076 | - |
RRApTVA | 25°C, pH 7 | chicken anemia virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| chicken anemia virus | - |
- |
- |
| chicken anemia virus VP2 | - |
- |
- |
| TTV-like mini virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
chicken anemia virus |
- |
TTV-like mini virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DADEpYLPQQG + H2O | - |
chicken anemia virus | DADEYLPQQG + phosphate | - |
? | |
| DADEpYLPQQG + H2O | - |
chicken anemia virus VP2 | DADEYLPQQG + phosphate | - |
? | |
| ENDpYINASL + H2O | - |
chicken anemia virus | ENDYINASL + phosphate | - |
? | |
| ENDpYINASL + H2O | - |
chicken anemia virus VP2 | ENDYINASL + phosphate | - |
? | |
| RRApTVA + H2O | - |
chicken anemia virus | RRATVA + phosphate | - |
? | |
| RRApTVA + H2O | - |
TTV-like mini virus | RRATVA + phosphate | - |
? | |
| RRApTVA + H2O | - |
chicken anemia virus VP2 | RRATVA + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAV VP2 | - |
chicken anemia virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
chicken anemia virus |
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Release 2023.1 (January 2023)
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