Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21DE3 codon+ cells in unsoluble inclusion bodies | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
p-nitrophenyl phosphate | purified, catalytically active 40 kDa-subunit, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C | Saccharomyces cerevisiae | |
34 | - |
p-nitrophenyl phosphate | full-length Ppt1, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for activity | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 4000 + 1 * 20000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-IDA column chromatography | Saccharomyces cerevisiae |
Renatured (Comment) | Organism |
---|---|
by stepwise dilution in 700 mM Tris pH 7.5, 250 mM arginine at 4°C, at a final protein concentration of 0.1 mg/ml | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Saccharomyces cerevisiae | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 4000 + 1 * 20000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PPT1 | - |
Saccharomyces cerevisiae |
protein phosphatase 1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
p-nitrophenyl phosphate | full-length Ppt1, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C | Saccharomyces cerevisiae | |
0.78 | - |
p-nitrophenyl phosphate | purified, catalytically active 40 kDa-subunit, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C | Saccharomyces cerevisiae |