Any feedback?
Literature summary for 3.1.3.16 extracted from
- The nonconserved N-terminus of protein phosphatase 2B confers its properties to protein phosphatase 1 (2009), IUBMB Life, 61, 178-183.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| chlorogenic acid | PP2B but not PP1 is stimulated 4fold by 0.1 mM chlorogenic acid | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the catalytic subunit of calcineurin is expressed in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the substitution mutant CNA(1-42)-PP1(9-330) has amino acids 1-42 of calcineurin A fused to truncated protein phosphatase 1 with N-terminal residues 1-8 deletion, the mutant is activated by both Mn2+ and Ni2+ and stimulated by chlorogenic acid | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | isozyme PP1 has low activity after depletion of Mn2+ with EDTA | Homo sapiens | |
| additional information | protein phosphatase 2B is highly resistant to inhibition by okadaic acid | Homo sapiens | |
| okadaic acid | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | isozyme PP1 activity is dependent on Mn2+ with 2.1fold stimulation at 3 mM | Homo sapiens | |
| Ni2+ | PP2B but not PP1 is stimulated by Ni2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
protein phosphatase 1, SDS-PAGE | Homo sapiens |
| 61000 | - |
recombinant catalytic subunit of calcineurin, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
| phosphoprotein RII + H2O | - |
Homo sapiens | protein RII + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| calcineurin | - |
Homo sapiens |
| CNA | calcineurin catalytic subunit | Homo sapiens |
| PP1 | - |
Homo sapiens |
| PP1c | protein phosphatase 1 catalytic subunit | Homo sapiens |
| PP2B | - |
Homo sapiens |
| protein phosphatase 1 | - |
Homo sapiens |
| protein phosphatase 2B | - |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
protein phosphatase 1 | Homo sapiens | okadaic acid | |
| 0.00012 | - |
mutant enzyme CNA(1-42)-PP1(9-330) | Homo sapiens | okadaic acid | |
| 0.005 | - |
protein phosphatase 2B | Homo sapiens | okadaic acid | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
