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Literature summary for 3.1.3.16 extracted from

  • Zuo, Y.; Selvakumar, P.; Sharma, R.K.
    Molecular cloning and biochemical characterization of bovine retina calcineurin (2010), Mol. Cell. Biochem., 333, 73-82.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
cDNA sequence determination and analysis, a 10 amino acid, residues ATVEAIEADE, deletion before the autoinhibitory domain is observed in bovine retina CaN A compared to bovine brain CaN A, sequence comparisona, expression of His-tagged isozymes in Escherichia coli strain M13(pREP4) using expression vector pQE9 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
additional information the residues at 407-456 also have an inhibitory effect on CaN A phosphatase activity in addition to the previously known autoinhibitory domain at 457-480 Bos taurus
Zn2+
-
Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Bos taurus
Fe2+ activates the eye isozyme, but might inhibit the brain isozyme Bos taurus
Mn2+ slight stimulation Bos taurus
Ni2+ strong activation Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE Bos taurus
60000
-
1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P48452 tissue-specific isozymes in eye and brain
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes from Escherichia coli strain M13 by nickel affinity chromatography to near homogeneity Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-
eye CaN is present in all eye tissues Bos taurus
-
retina
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Bos taurus 4-nitrophenol + phosphate
-
?
additional information residues at 97-201 are essential for bovine retina CaN subunit A phosphatase activity Bos taurus ?
-
?
phosphorylated histone + H2O
-
Bos taurus histone + phosphate
-
?

Subunits

Subunits Comment Organism
heterotrimer 1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
calcineurin
-
Bos taurus
CaN
-
Bos taurus
CaN A phosphatase
-
Bos taurus
More calcineurin is a member of the Ser/Thr protein phosphatase family Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bos taurus