Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Bos taurus |
Cloned (Comment) | Organism |
---|---|
cDNA sequence determination and analysis, a 10 amino acid, residues ATVEAIEADE, deletion before the autoinhibitory domain is observed in bovine retina CaN A compared to bovine brain CaN A, sequence comparisona, expression of His-tagged isozymes in Escherichia coli strain M13(pREP4) using expression vector pQE9 | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the residues at 407-456 also have an inhibitory effect on CaN A phosphatase activity in addition to the previously known autoinhibitory domain at 457-480 | Bos taurus | |
Zn2+ | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Bos taurus | |
Fe2+ | activates the eye isozyme, but might inhibit the brain isozyme | Bos taurus | |
Mn2+ | slight stimulation | Bos taurus | |
Ni2+ | strong activation | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE | Bos taurus |
60000 | - |
1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P48452 | tissue-specific isozymes in eye and brain | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes from Escherichia coli strain M13 by nickel affinity chromatography to near homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
eye | CaN is present in all eye tissues | Bos taurus | - |
retina | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Bos taurus | 4-nitrophenol + phosphate | - |
? | |
additional information | residues at 97-201 are essential for bovine retina CaN subunit A phosphatase activity | Bos taurus | ? | - |
? | |
phosphorylated histone + H2O | - |
Bos taurus | histone + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
calcineurin | - |
Bos taurus |
CaN | - |
Bos taurus |
CaN A phosphatase | - |
Bos taurus |
More | calcineurin is a member of the Ser/Thr protein phosphatase family | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bos taurus |