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Literature summary for 3.1.3.16 extracted from

  • Reimann, J.; Esser, D.; Orell, A.; Amman, F.; Pham, T.K.; Noirel, J.; Lindas A.C.; Bernander, R.; Wright, P.C.; Siebers, B.; Albers, S.V.
    Archaeal signal transduction: impact of protein phosphatase deletions on cell size, motility, and energy metabolism in Sulfolobus acidocaldarius (2013), Mol. Cell. Proteomics, 12, 3908-3923.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta (DE3)-RIL Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
okadaic acid 56% activity in presence of 10 nM okadaic acid Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0536
-
 RRA(pT)VA pH 6.5, 70°C Sulfolobus acidocaldarius
0.423
-
 NIDAIRA(pS)LNIMSR pH 6.5, 70°C Sulfolobus acidocaldarius
0.49
-
 ETTYERW(pT)TITQRER pH 6.5, 70°C Sulfolobus acidocaldarius
0.53
-
 4-nitrophenyl phosphate pH 6.5, 70°C Sulfolobus acidocaldarius
2.5
-
 4-nitrophenyl phosphate pH 6.5, 70°C Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
Co2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
Mg2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
Mn2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
Ni2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-L-serine phosphate + H2O Sulfolobus acidocaldarius
-
 [protein]-L-serine + phosphate
-
 ?
[protein]-L-serine phosphate + H2O Sulfolobus acidocaldarius MW001
-
 [protein]-L-serine + phosphate
-
 ?
[protein]-L-threonine phosphate + H2O Sulfolobus acidocaldarius
-
 [protein]-L-threonine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4JAD1 the wild type strain DSM 639 missing 322 bp called MW001
-
Sulfolobus acidocaldarius Q4JB88 the wild type strain DSM 639 missing 322 bp called MW001
-
Sulfolobus acidocaldarius ATCC 33909 Q4JAD1 the wild type strain DSM 639 missing 322 bp called MW001
-
Sulfolobus acidocaldarius MW001 Q4JB88 the wild type strain DSM 639 missing 322 bp called MW001
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius MW001 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius ATCC 33909 4-nitrophenol + phosphate
-
 ?
ETTYERW(pT)TITQRER + H2O
-
 Sulfolobus acidocaldarius ETTYERWTTITQRER + phosphate
-
 ?
additional information no activity with TEVGKRI(pY)RLVGDKN Sulfolobus acidocaldarius ?
-
 ?
additional information no activity with TEVGKRI(pY)RLVGDKN Sulfolobus acidocaldarius ATCC 33909 ?
-
 ?
NIDAIRA(pS)LNIMSR + H2O
-
 Sulfolobus acidocaldarius NIDAIRASLNIMSR + phosphate
-
 ?
RRA(pT)VA + H2O
-
 Sulfolobus acidocaldarius RRATVA + phosphate
-
 ?
RRA(pT)VA + H2O
-
 Sulfolobus acidocaldarius ATCC 33909 RRATVA + phosphate
-
 ?
[protein]-L-serine phosphate + H2O
-
 Sulfolobus acidocaldarius [protein]-L-serine + phosphate
-
 ?
[protein]-L-serine phosphate + H2O
-
 Sulfolobus acidocaldarius MW001 [protein]-L-serine + phosphate
-
 ?
[protein]-L-threonine phosphate + H2O
-
 Sulfolobus acidocaldarius [protein]-L-threonine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Saci-PP2A
-
 Sulfolobus acidocaldarius
Saci-PTP dual specific phosphatase EC 3.1.3.16/EC 3.1.3.48 Sulfolobus acidocaldarius
Saci0545 locus name, dual specific phosphatase EC 3.1.3.16/EC 3.1.3.48 Sulfolobus acidocaldarius
Saci0884
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Sulfolobus acidocaldarius