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Literature summary for 3.1.3.16 extracted from

  • Kant, S.; Agarwal, S.; Pancholi, P.; Pancholi, V.
    The Streptococcus pyogenes orphan protein tyrosine phosphatase, SP-PTP, possesses dual specificity and essential virulence regulatory functions (2015), Mol. Microbiol., 97, 515-540 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene SPy_0036, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Streptococcus pyogenes serotype M28
gene SPy_0039, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Streptococcus pyogenes serotype M1

Protein Variants

Protein Variants Comment Organism
C8A site-directed mutagenesis, inactive enzyme mutant of SP-PTP Streptococcus pyogenes serotype M1
additional information A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of a M1T1DELTAPTP enzyme knockout mutant, phenotype, overview Streptococcus pyogenes serotype M1
additional information A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of Streptoccocus pyogenes mutant lacking the ptp-(Spy_0036) gene derived from the Type M1 M1T15448 strain by allelic replacement Streptococcus pyogenes serotype M28
R14A site-directed mutagenesis, the enzyme mutant of SP-PTP shows highly reduced activity compared to wild-type Streptococcus pyogenes serotype M1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten and Lineweaver-Burk kinetics Streptococcus pyogenes serotype M1
additional information
-
additional information Michaelis-Menten and Lineweaver-Burk kinetics Streptococcus pyogenes serotype M28

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Streptococcus pyogenes serotype M1
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[a protein]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M1
-
[a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M28
-
[a protein]-serine/threonine + phosphate
-
?
[SP-STK kinase]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M1
-
[SP-STK kinase]-serine/threonine + phosphate
-
?
[SP-STK kinase]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M28
-
[SP-STK kinase]-serine/threonine + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes serotype M1 Q9A1Y0 MGAS5005 genome
-
Streptococcus pyogenes serotype M28 Q48VV6 M1SF370 genome
-
Streptococcus pyogenes serotype M28 MGAS6180 Q48VV6 M1SF370 genome
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Streptococcus pyogenes serotype M1
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Streptococcus pyogenes serotype M28

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Streptococcus pyogenes serotype M1 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Streptococcus pyogenes serotype M28 4-nitrophenol + phosphate
-
?
additional information SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M1 ?
-
?
additional information SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M28 ?
-
?
additional information SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M28 MGAS6180 ?
-
?
[a protein]-serine/threonine phosphate + H2O
-
Streptococcus pyogenes serotype M1 [a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O
-
Streptococcus pyogenes serotype M28 [a protein]-serine/threonine + phosphate
-
?
[SP-STK kinase]-serine/threonine phosphate + H2O
-
Streptococcus pyogenes serotype M1 [SP-STK kinase]-serine/threonine + phosphate
-
?
[SP-STK kinase]-serine/threonine phosphate + H2O
-
Streptococcus pyogenes serotype M28 [SP-STK kinase]-serine/threonine + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE Streptococcus pyogenes serotype M1
? x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE Streptococcus pyogenes serotype M28

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.3.48 Streptococcus pyogenes serotype M1
More cf. EC 3.1.3.48 Streptococcus pyogenes serotype M28
SP-PTP
-
Streptococcus pyogenes serotype M1
SPy_0036
-
Streptococcus pyogenes serotype M28
SPy_0039
-
Streptococcus pyogenes serotype M1

Expression

Organism Comment Expression
Streptococcus pyogenes serotype M1 the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell up
Streptococcus pyogenes serotype M28 the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell up

General Information

General Information Comment Organism
evolution group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) Streptococcus pyogenes serotype M1
evolution group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) Streptococcus pyogenes serotype M28
malfunction absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morphology, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype Streptococcus pyogenes serotype M1
malfunction absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morphology, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype Streptococcus pyogenes serotype M28
physiological function SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence Streptococcus pyogenes serotype M1
physiological function SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence Streptococcus pyogenes serotype M28