Cloned (Comment) | Organism |
---|---|
gene SPy_0036, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Streptococcus pyogenes serotype M28 |
gene SPy_0039, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Streptococcus pyogenes serotype M1 |
Protein Variants | Comment | Organism |
---|---|---|
C8A | site-directed mutagenesis, inactive enzyme mutant of SP-PTP | Streptococcus pyogenes serotype M1 |
additional information | A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of a M1T1DELTAPTP enzyme knockout mutant, phenotype, overview | Streptococcus pyogenes serotype M1 |
additional information | A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of Streptoccocus pyogenes mutant lacking the ptp-(Spy_0036) gene derived from the Type M1 M1T15448 strain by allelic replacement | Streptococcus pyogenes serotype M28 |
R14A | site-directed mutagenesis, the enzyme mutant of SP-PTP shows highly reduced activity compared to wild-type | Streptococcus pyogenes serotype M1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten and Lineweaver-Burk kinetics | Streptococcus pyogenes serotype M1 | |
additional information | - |
additional information | Michaelis-Menten and Lineweaver-Burk kinetics | Streptococcus pyogenes serotype M28 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Streptococcus pyogenes serotype M1 | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-serine/threonine phosphate + H2O | Streptococcus pyogenes serotype M1 | - |
[a protein]-serine/threonine + phosphate | - |
? | |
[a protein]-serine/threonine phosphate + H2O | Streptococcus pyogenes serotype M28 | - |
[a protein]-serine/threonine + phosphate | - |
? | |
[SP-STK kinase]-serine/threonine phosphate + H2O | Streptococcus pyogenes serotype M1 | - |
[SP-STK kinase]-serine/threonine + phosphate | - |
? | |
[SP-STK kinase]-serine/threonine phosphate + H2O | Streptococcus pyogenes serotype M28 | - |
[SP-STK kinase]-serine/threonine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes serotype M1 | Q9A1Y0 | MGAS5005 genome | - |
Streptococcus pyogenes serotype M28 | Q48VV6 | M1SF370 genome | - |
Streptococcus pyogenes serotype M28 MGAS6180 | Q48VV6 | M1SF370 genome | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Streptococcus pyogenes serotype M1 |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Streptococcus pyogenes serotype M28 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Streptococcus pyogenes serotype M1 | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Streptococcus pyogenes serotype M28 | 4-nitrophenol + phosphate | - |
? | |
additional information | SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK | Streptococcus pyogenes serotype M1 | ? | - |
? | |
additional information | SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK | Streptococcus pyogenes serotype M28 | ? | - |
? | |
additional information | SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK | Streptococcus pyogenes serotype M28 MGAS6180 | ? | - |
? | |
[a protein]-serine/threonine phosphate + H2O | - |
Streptococcus pyogenes serotype M1 | [a protein]-serine/threonine + phosphate | - |
? | |
[a protein]-serine/threonine phosphate + H2O | - |
Streptococcus pyogenes serotype M28 | [a protein]-serine/threonine + phosphate | - |
? | |
[SP-STK kinase]-serine/threonine phosphate + H2O | - |
Streptococcus pyogenes serotype M1 | [SP-STK kinase]-serine/threonine + phosphate | - |
? | |
[SP-STK kinase]-serine/threonine phosphate + H2O | - |
Streptococcus pyogenes serotype M28 | [SP-STK kinase]-serine/threonine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE | Streptococcus pyogenes serotype M1 |
? | x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE | Streptococcus pyogenes serotype M28 |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.48 | Streptococcus pyogenes serotype M1 |
More | cf. EC 3.1.3.48 | Streptococcus pyogenes serotype M28 |
SP-PTP | - |
Streptococcus pyogenes serotype M1 |
SPy_0036 | - |
Streptococcus pyogenes serotype M28 |
SPy_0039 | - |
Streptococcus pyogenes serotype M1 |
Organism | Comment | Expression |
---|---|---|
Streptococcus pyogenes serotype M1 | the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell | up |
Streptococcus pyogenes serotype M28 | the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell | up |
General Information | Comment | Organism |
---|---|---|
evolution | group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) | Streptococcus pyogenes serotype M1 |
evolution | group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) | Streptococcus pyogenes serotype M28 |
malfunction | absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morphology, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype | Streptococcus pyogenes serotype M1 |
malfunction | absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morphology, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype | Streptococcus pyogenes serotype M28 |
physiological function | SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence | Streptococcus pyogenes serotype M1 |
physiological function | SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence | Streptococcus pyogenes serotype M28 |