Literature summary for 3.1.3.17 extracted from

Gratecos, D.; Detwiler, T.C.; Hurd, S.; Fischer, E.H.
Rabbit muscle phosphorylase phosphatase. 1. Purification and chemical properties (1977), Biochemistry, 16, 4812-4817.

Search for more literature for 3.1.3.17

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	maximal activation at 50 mM, absolute requirement for SH compounds	Oryctolagus cuniculus
ascorbic acid	maximal activation at 1 mM	Oryctolagus cuniculus
dithiothreitol	maximal activation at 1 mM, absolute requirement for SH compounds	Oryctolagus cuniculus

General Stability

General Stability	Organism
complete inactivation after treatment with 0.3 M perchloric acid or 10% trichloroacetic acid	Oryctolagus cuniculus
complete inactivation after treatment with 1% dodecyl sulfate or 0.05% cetyltrimethylammonium bromide or 1 M guanidine-HCl after 15 min	Oryctolagus cuniculus
complete inactivation after treatment with 10% w/w pronase at 30°C for 3 h	Oryctolagus cuniculus
resistant to trypsin and chymotrypsin and to 8 M urea	Oryctolagus cuniculus

Inhibitors

Inhibitors	Comment	Organism	Structure
2-hydroxyethyl disulfide	-	Oryctolagus cuniculus
5,5'-dithiobis(2-nitrobenzoic acid)	-	Oryctolagus cuniculus
Ca2+	10 mM, 20% inhibition	Oryctolagus cuniculus
cystamine	-	Oryctolagus cuniculus
cystine	-	Oryctolagus cuniculus
D-Pantethine	-	Oryctolagus cuniculus
Disulfides	irreversible inactivation	Oryctolagus cuniculus
GSSG	-	Oryctolagus cuniculus
iodoacetamide	50 mM, 30% inhibition after 1 h at 30°C	Oryctolagus cuniculus
iodoacetic acid	50 mM, 30% inhibition after 1 h at 30°C	Oryctolagus cuniculus
Mg2+	10 mM, 20% inhibition	Oryctolagus cuniculus
Mn2+	3 mM, 64% inhibition	Oryctolagus cuniculus
NaCl	0.1-0.3 M, 40-96% inhibition	Oryctolagus cuniculus
poly(L-lysine)	0.001 mM, strong	Oryctolagus cuniculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	inactive in absence of Mn2+	Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	gel filtration	Oryctolagus cuniculus

Organic Solvent Stability

Organic Solvent	Comment	Organism
deoxycholate	stable to 0.5% deoxycholate, 24 h	Oryctolagus cuniculus
Ethanol	stable to 25% ethanol at -5°C, 3 h	Oryctolagus cuniculus
Triton X-100	stable to 0.5% Triton X-100, 24 h	Oryctolagus cuniculus
Tween	stable to 0.1% Tween 20, 40 or 80, 24 h	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no modification	the enzyme is free of bound phosphate, carbohydrate, lipids and nucleic acids	Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment)	Organism
-	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1	-	-	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphorylase a + H2O	-	Oryctolagus cuniculus	phosphorylase b + phosphate	-	?
troponin I + H2O	-	Oryctolagus cuniculus	phosphate + ?	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	15 min, 50% loss of activity	Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Oryctolagus cuniculus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8.7	about 50% of maximal activity at pH 6.5 and at pH 8.7	Oryctolagus cuniculus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	-	30 min, 90% loss of activity	Oryctolagus cuniculus

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Release 2023.1 (January 2023)