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Literature summary for 3.1.3.18 extracted from
- Characteristics and sequence of phosphoglycolate phosphatase from a eukaryotic green alga Chlamydomonas reinhardtii (2001), J. Biol. Chem., 276, 45573-45579.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | the enzyme binds to Ca2+, the inhibition is recovered by addition of EGTA and MgCl2 | Chlamydomonas reinhardtii |  |
| p-mercuribenzoic acid | the inhibition is recovered by addition of dithiothreitol | Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
2-Phosphoglycolate | pH 8.0 | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Chlamydomonas reinhardtii |
| 65000 | - |
gelfiltration | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Phosphoglycolate + H2O | Chlamydomonas reinhardtii | a key enzyme of photorespiration in photosynthetic organisms | Glycolate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O67359 | - |
- |
| Chlamydomonas reinhardtii | Q948T5 | eukaryotic green alga | - |
| Cupriavidus necator | P40852 | - |
- |
| Escherichia coli | P32662 | - |
- |
| Synechocystis sp. | P73525 | PCC6803 | - |
| Triticum aestivum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 86.5 | - |
- |
Chlamydomonas reinhardtii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable at least 6 months | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Phosphoglycolate + H2O | - |
Chlamydomonas reinhardtii | Glycolate + phosphate | - |
? | |
| 2-Phosphoglycolate + H2O | a key enzyme of photorespiration in photosynthetic organisms | Chlamydomonas reinhardtii | Glycolate + phosphate | - |
? | |
| D-fructose-1,6-bisphosphate + H2O | - |
Chlamydomonas reinhardtii | D-fructose-1-phosphate + phosphate | - |
? | |
| D-ribulose-5-phosphate + H2O | - |
Chlamydomonas reinhardtii | D-ribulose + phosphate | - |
? | |
| additional information | not: 3-phosphoglycerate, D-ribose-5-phosphate, phosphoenolpyruvate, O-phospho-1-serine, p-nitrophenylphosphate | Chlamydomonas reinhardtii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 32000, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGPase | - |
Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | 9 | - |
Chlamydomonas reinhardtii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Chlamydomonas reinhardtii | isoelectric focusing | - |
5.1 |
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Release 2023.1 (January 2023)
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