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Literature summary for 3.1.3.2 extracted from
- Properties of two membrane-bound acid phosphatases compared with those of a cytoplasmic acid phosphatase from Dioscorea cayenensis rotundata (1983), Plant Sci. Lett., 32, 305-312.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM | Dioscorea cayenensis-rotundata |  |
| EDTA | - |
Dioscorea cayenensis-rotundata | |
| Hg2+ | - |
Dioscorea cayenensis-rotundata | |
| molybdate | - |
Dioscorea cayenensis-rotundata | |
| Na+ | 10 mM | Dioscorea cayenensis-rotundata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
p-nitrophenyl phosphate | enzyme A | Dioscorea cayenensis-rotundata | |
| 3.1 | - |
p-nitrophenyl phosphate | enzyme B | Dioscorea cayenensis-rotundata | |
| 3.4 | - |
p-nitrophenyl phosphate | enzyme C | Dioscorea cayenensis-rotundata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | acid phosphatase A | Dioscorea cayenensis-rotundata | 5737 | - |
| membrane | acid phosphatase B and C | Dioscorea cayenensis-rotundata | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activates | Dioscorea cayenensis-rotundata | |
| Mg2+ | stimulates | Dioscorea cayenensis-rotundata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
1 * 50000, enzyme B, SDS-PAGE | Dioscorea cayenensis-rotundata |
| 55000 | - |
enzyme B, gel filtration | Dioscorea cayenensis-rotundata |
| 56000 | - |
1 * 56000, enzyme C, SDS-PAGE | Dioscorea cayenensis-rotundata |
| 65000 | - |
enzyme C, gel filtration | Dioscorea cayenensis-rotundata |
| 98000 | - |
cytoplasmic enzyme A, gel filtration | Dioscorea cayenensis-rotundata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dioscorea cayenensis-rotundata | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Dioscorea cayenensis-rotundata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membrane bound enzymes B and C | Dioscorea cayenensis-rotundata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1100 | - |
- |
Dioscorea cayenensis-rotundata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl phosphate + H2O | - |
Dioscorea cayenensis-rotundata | 1-naphthol + phosphate | - |
? | |
| 2-glycerophosphate + H2O | enzyme B and C, no activity with enzyme A | Dioscorea cayenensis-rotundata | glycerol + phosphate | - |
? | |
| 2-naphthyl phosphate + H2O | - |
Dioscorea cayenensis-rotundata | 2-naphthol + phosphate | - |
? | |
| 5'-AMP + H2O | - |
Dioscorea cayenensis-rotundata | adenosine + phosphate | - |
? | |
| ADP + H2O | - |
Dioscorea cayenensis-rotundata | AMP + phosphate | - |
? | |
| alpha-glycerophosphate + H2O | enzyme B and C, no activity with enzyme A | Dioscorea cayenensis-rotundata | glycerol + phosphate | - |
? | |
| ATP + H2O | enzyme B and C, no activity with enzyme A | Dioscorea cayenensis-rotundata | ADP + phosphate | - |
? | |
| D-fructose 1,6-diphosphate + H2O | enzyme B and C, no activity with enzyme A | Dioscorea cayenensis-rotundata | ? | - |
? | |
| D-fructose 6-phosphate + H2O | enzyme B and C, no activity with enzyme A | Dioscorea cayenensis-rotundata | D-fructose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | - |
Dioscorea cayenensis-rotundata | D-ribose + phosphate | - |
? | |
| glucose 1-phosphate + H2O | enzyme B and C, no activity with enzyme A | Dioscorea cayenensis-rotundata | glucose + phosphate | - |
? | |
| Glucose 6-phosphate + H2O | enzyme B and C, no activity with enzyme A | Dioscorea cayenensis-rotundata | Glucose + phosphate | - |
? | |
| glyceraldehyde-3-phosphoric acid diethylacetal + H2O | - |
Dioscorea cayenensis-rotundata | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Dioscorea cayenensis-rotundata | p-nitrophenol + phosphate | - |
? | |
| phenyl phosphate + H2O | enzyme A, no activity with enzyme B and C | Dioscorea cayenensis-rotundata | phenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 56000, enzyme C, SDS-PAGE | Dioscorea cayenensis-rotundata |
| monomer | 1 * 50000, enzyme B, SDS-PAGE | Dioscorea cayenensis-rotundata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
enzyme B | Dioscorea cayenensis-rotundata |
| 5 | - |
enzyme A | Dioscorea cayenensis-rotundata |
| 5.2 | - |
enzyme C | Dioscorea cayenensis-rotundata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | stable | Dioscorea cayenensis-rotundata |
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