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Literature summary for 3.1.3.2 extracted from

  • Harsanyi, Z.; Dorn, G.L.
    Purification and characterization of acid phosphatase V from Aspergillus nidulans (1972), J. Bacteriol., 110, 246-255.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Borate
-
Aspergillus nidulans
KCl
-
Aspergillus nidulans
molybdate
-
Aspergillus nidulans
NaCl
-
Aspergillus nidulans
phosphate
-
Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
p-nitrophenyl phosphate
-
Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus nidulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1960
-
-
Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O 68% of the activity with p-nitrophenyl phosphate Aspergillus nidulans adenosine + phosphate
-
?
5'-AMP + H2O 72% of the activity with p-nitrophenyl phosphate Aspergillus nidulans adenosine + phosphate
-
?
5'-CMP + H2O 46% of the activity with p-nitrophenyl phosphate Aspergillus nidulans cytidine + phosphate
-
?
5'-dAMP + H2O
-
Aspergillus nidulans deoxyadenosine + phosphate
-
?
5'-GMP + H2O 52% of the activity with p-nitrophenyl phosphate Aspergillus nidulans guanosine + phosphate
-
?
5'-UMP + H2O
-
Aspergillus nidulans uridine + phosphate
-
?
Fructose 1-phosphate + H2O 52% of the activity with p-nitrophenyl phosphate Aspergillus nidulans Fructose + phosphate
-
?
glucose 1-phosphate + H2O 52% of the activity with p-nitrophenyl phosphate Aspergillus nidulans glucose + phosphate
-
?
Glucose 6-phosphate + H2O
-
Aspergillus nidulans Glucose + phosphate
-
?
O-phosphoserine + H2O 69% of maximal activity Aspergillus nidulans Ser + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Aspergillus nidulans p-nitrophenol + phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life: 24 min Aspergillus nidulans
65
-
half-life: 30 sec Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
-
Aspergillus nidulans

pH Range

pH Minimum pH Maximum Comment Organism
5.2 7 pH 5.2: about 50% of maximal activity, pH 7.0: about 15% of maximal activity Aspergillus nidulans