Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10 mM, activation to 156% of control | Latilactobacillus curvatus | |
EDTA | 10 mM, activation to 171% of control | Latilactobacillus curvatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 27% inhibition | Latilactobacillus curvatus | |
BaCl2 | 10 mM, 16% inhibition | Latilactobacillus curvatus | |
CaCl2 | 10 mM, 15% inhibition | Latilactobacillus curvatus | |
Hexametaphosphate | 10 mM, 44% inhibition | Latilactobacillus curvatus | |
HgCl2 | 10 mM, 83% inhibition | Latilactobacillus curvatus | |
MnCl2 | 10 mM, 65% inhibition | Latilactobacillus curvatus | |
NaF | 0.1 mM, 88% inhibition, complete inhibition at 1 mM | Latilactobacillus curvatus | |
PCMB | 10 mM, 35% inhibition | Latilactobacillus curvatus | |
PMSF | 10 mM, 85% inhibition | Latilactobacillus curvatus | |
tripolyphosphate | 10 mM, 54% inhibition | Latilactobacillus curvatus | |
ZnCl2 | 11 mM, 87% inhibition | Latilactobacillus curvatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 0.1 mM, activation to 130% of control | Latilactobacillus curvatus | |
MnCl2 | 0.1 mM, activation to 119% of control | Latilactobacillus curvatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
4 * 26000, SDS-PAGE | Latilactobacillus curvatus |
104000 | - |
gel filtration | Latilactobacillus curvatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Latilactobacillus curvatus | - |
- |
- |
Latilactobacillus curvatus DPC2024 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Latilactobacillus curvatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1675 | - |
- |
Latilactobacillus curvatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | 5% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | 1-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | 26% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | 2-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | 26% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus DPC2024 | 2-naphthol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Latilactobacillus curvatus | 4-nitrophenol + phosphate | - |
? | |
5'-ADP + H2O | 3% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | ? | - |
? | |
5'-ADP + H2O | 3% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus DPC2024 | ? | - |
? | |
5'-AMP + H2O | 13% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | adenosine + phosphate | - |
? | |
5'-AMP + H2O | 13% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus DPC2024 | adenosine + phosphate | - |
? | |
5'-ATP + H2O | 18% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | ? | - |
? | |
5'-ATP + H2O | 18% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus DPC2024 | ? | - |
? | |
alpha-D-glucose 1-phosphate + H2O | 9% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | alpha-D-glucose + phosphate | - |
? | |
alpha-D-glucose 6-phosphate + H2O | 58% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | alpha-D-glucose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | 87% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | D-fructose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | 87% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus DPC2024 | D-fructose + phosphate | - |
? | |
O-phospho-DL-serine + H2O | 16% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | ? | - |
? | |
O-phospho-DL-threonine + H2O | 5% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | ? | - |
? | |
O-phospho-DL-tyrosine + H2O | 4% of the activity with 4-nitrophenyl phosphate | Latilactobacillus curvatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 26000, SDS-PAGE | Latilactobacillus curvatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Latilactobacillus curvatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 30% of maximal activity, 80°C: about 40% of maximal activity | Latilactobacillus curvatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 4.5, 60 min, about 10% loss of activity | Latilactobacillus curvatus |
70 | - |
pH 4.5, 30 min, more than 65% of the activity is retained | Latilactobacillus curvatus |
75 | - |
pH 4.5, 30 min, about 80% loss of activity | Latilactobacillus curvatus |
80 | - |
pH 4.5, 10 min, about 80% loss of activity | Latilactobacillus curvatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Latilactobacillus curvatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | pH 4.0: about 60% of maximal activity, pH 6.0: about 35% of maximal activity | Latilactobacillus curvatus |