Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | mixed inhibition | Cucumis sativus | |
K+ | inhibitory at 10 mM | Cucumis sativus | |
additional information | not inhibited by Pb | Cucumis sativus | |
Zn2+ | competitive inhibition | Cucumis sativus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mn2+, Na+ and Cd2+ do not significantly alter the enzyme activity | Cucumis sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis sativus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Cucumis sativus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Cucumis sativus | ADP + phosphate | - |
? | |
beta-glycerol phosphate + H2O | - |
Cucumis sativus | glycerol + phosphate | - |
? | |
diphosphate + H2O | - |
Cucumis sativus | 2 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
orthophosphoric-monoester phosphohydrolase | - |
Cucumis sativus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.02 | - |
with ATP as substrate, in 3.5 mM sodium azide, 2.5 mM calcium chloride, 100 mM citrate buffer, pH 5.5, at 35°C | Cucumis sativus | Hg2+ | |
0.035 | - |
with diphosphate as substrate, in 3.5 mM sodium azide, 2.5 mM calcium chloride, 100 mM citrate buffer, pH 5.5, at 35°C | Cucumis sativus | Zn2+ | |
0.056 | - |
with ATP as substrate, in 3.5 mM sodium azide, 2.5 mM calcium chloride, 100 mM citrate buffer, pH 5.5, at 35°C | Cucumis sativus | Zn2+ | |
0.15 | - |
with beta-glycerol phosphate as substrate, in 3.5 mM sodium azide, 2.5 mM calcium chloride, 100 mM citrate buffer, pH 5.5, at 35°C | Cucumis sativus | Hg2+ | |
0.24 | - |
with beta-glycerol phosphate as substrate, in 3.5 mM sodium azide, 2.5 mM calcium chloride, 100 mM citrate buffer, pH 5.5, at 35°C | Cucumis sativus | Zn2+ | |
0.3 | - |
with diphosphate as substrate, in 3.5 mM sodium azide, 2.5 mM calcium chloride, 100 mM citrate buffer, pH 5.5, at 35°C | Cucumis sativus | Hg2+ |