General Stability | Organism |
---|---|
CaCl2 increases pH-stability and thermal stability | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Bacillus sp. (in: Bacteria) | |
Cd2+ | - |
Bacillus sp. (in: Bacteria) | |
Co2+ | - |
Bacillus sp. (in: Bacteria) | |
Cr3+ | - |
Bacillus sp. (in: Bacteria) | |
Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
EDTA | 1 mM, 94% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | - |
Bacillus sp. (in: Bacteria) | |
Mn2+ | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
myo-inositol hexakisphosphate | pH 7.0 | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
1 * 44000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
46000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Bacillus sp. (in: Bacteria) KHU-10 | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | phytase increases markedly at the late stationary phase | Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP | Bacillus sp. (in: Bacteria) KHU-10 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | very specific for, no activity on other phosphate esters | Bacillus sp. (in: Bacteria) | ? + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | very specific for, no activity on other phosphate esters | Bacillus sp. (in: Bacteria) KHU-10 | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
without Ca2+ | Bacillus sp. (in: Bacteria) |
60 | - |
in presence of 10 mM CaCl2 | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 30°C: about 40% of maximal activity, 80°C: about 55% of maximal activity, presence of 10 mM CaCl2 | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, in presence of 10 mM CaCl2, stable up to | Bacillus sp. (in: Bacteria) |
70 | - |
10 min, in presence of 10 mM CaCl2, 35% loss of activity | Bacillus sp. (in: Bacteria) |
80 | - |
10 min, in presence of 10 mM CaCl2, 50% loss of activity | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.6 | - |
myo-inositol hexakisphosphate | pH 7.0 | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | in presence of 10 mM CaCl2 | Bacillus sp. (in: Bacteria) |
6.5 | 8.5 | without Ca2+ | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0: about 50% of maximal activity, pH 10.0: about 80% of maximal activity, presence of 10 mM CaCl2 | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | isoelectric focusing | - |
6.8 |