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Literature summary for 3.1.3.26 extracted from

  • Choi, Y.M.; Suh, H.J.; Kim, J.M.
    Purification and properties of extracellular phytase from Bacillus sp. KHU-10 (2001), J. Protein Chem., 20, 287-292.
    View publication on PubMed
Search for more literature for 3.1.3.26

General Stability

General Stability Organism
CaCl2 increases pH-stability and thermal stability Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Bacillus sp. (in: Bacteria)
Cd2+
-
 Bacillus sp. (in: Bacteria)
Co2+
-
 Bacillus sp. (in: Bacteria)
Cr3+
-
 Bacillus sp. (in: Bacteria)
Cu2+
-
 Bacillus sp. (in: Bacteria)
EDTA 1 mM, 94% inhibition Bacillus sp. (in: Bacteria)
Hg2+
-
 Bacillus sp. (in: Bacteria)
Mn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 myo-inositol hexakisphosphate pH 7.0 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 1 * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)
46000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Bacillus sp. (in: Bacteria) KHU-10
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
cell culture phytase increases markedly at the late stationary phase Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) KHU-10 ?
-
 ?
myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) KHU-10 ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 without Ca2+ Bacillus sp. (in: Bacteria)
60
-
 in presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 40% of maximal activity, 80°C: about 55% of maximal activity, presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, in presence of 10 mM CaCl2, stable up to Bacillus sp. (in: Bacteria)
70
-
 10 min, in presence of 10 mM CaCl2, 35% loss of activity Bacillus sp. (in: Bacteria)
80
-
 10 min, in presence of 10 mM CaCl2, 50% loss of activity Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.6
-
 myo-inositol hexakisphosphate pH 7.0 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9.5 in presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)
6.5 8.5 without Ca2+ Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 50% of maximal activity, pH 10.0: about 80% of maximal activity, presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) isoelectric focusing
-
 6.8